LOCUS       sequence-87238-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1381..1383,1384..1386,1387..2100)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1426..1447)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1687..1706)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2034..2055
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgaga ccgccatggc cactgttacc
       61 tatccttcat ctgagcctac gaccttggac cctgggaacg catcctcgac ctggcccctg
      121 gataccaccc tggggaacac atccgctggc gctagcctga caggcctggc tgtcagtggc
      181 atcttgatct ctctggtgta cctggtggtg tgcgtggtgg gtttgctggg caactcgctg
      241 gtgatctacg tggtcctgcg gcacacgtcc agcccatcag tgaccagtgt ctatatcctc
      301 aacctggctc tggctgatga gctcttcatg ctagggctac ccttcctggc tgctcagaac
      361 gccctgtcct actggccctt cggatctctc atgtgccgtc tggtcatggc cgtggatggc
      421 atcaaccagt tcaccagcat cttctgcctc accgtcatga gtgtggaccg ctatctggct
      481 gtggtgcacc ccacacgctc agcccgctgg cgcacggcac cagtggctcg cacggtcagt
      541 gcagctgtct gggtggcctc ggctgtggtg gtgctgcctg tggttgtgtt ctcaggagtg
      601 ccccggggca tgagcacgtg ccacatgcag tggccagagc cagcggctgc ctggcgaaca
      661 gccttcatca tctacacggc cgcactgggc ttctttgggc ccctgctggt catctgcttg
      721 tgctacttgc tcattgtggt aaaggtgcgg tcgaccaccc ggcgggtgcg ggcgccctcg
      781 tgtcagtggg tacaggcacc cgcatgccag cggcgacgcc gctctgagcg cagggtcaca
      841 cgcatggtgg tggccgtggt ggcactcttc gtcctctgct ggatgccttt ctatctgctc
      901 aacatcgtca atgtggtgtg cccgctgccg gaggagcccg ccttcttcgg cctctacttc
      961 ctggtggtgg cgctgcccta cgccaacagc tgcgcaaacc ccatcctcta cggcttcctc
     1021 tcctaccgct tcaagcaggg ctttcgcagg atcctgctaa gaccatcacg tcgcattcgg
     1081 agccaggagc cagggtcggg acctccagag aagactgaag aggaggagga tgaagaagaa
     1141 gaagagagaa gggaagagga ggagcggagg atgcagagag ggcaggagat gaacgggagg
     1201 ctcagtcaga tcgcacaggc tggcactagt ggacaacagc cacggccctg cacagggact
     1261 gctaaggagc agcagcttct gccccaggag gccacagctg gggacaaggc cagcacactg
     1321 agccatctgg gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
     1381 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1441 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1501 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1561 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     1621 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1681 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1741 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1801 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
     1861 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     1921 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1981 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2041 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     2101 agcggccgcg actctagatc ataatcagcc
//
