LOCUS       sequence-87239-e        8478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8478)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Talin-N-10
FEATURES             Location/Qualifiers
     source          1..8478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7714..7723
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7720..7722,7723..7725,7726..8439)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7765..7786)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8026..8045)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8373..8394
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcta
       61 gccaccatgg ttgcgctttc gctgaagatt agcattggga atgtggtgaa gacgatgcaa
      121 tttgagccat ctaccatggt gtacgacgcc tgccgcatga ttcgtgagcg gatcccagag
      181 gccctggctg gccctcccaa cgactttggg ctctttctgt cagatgatga ccccaaaaaa
      241 ggcatctggc tagaggctgg gaaagctttg gactactaca tgctccgaaa tggggacacc
      301 atggagtaca gaaaaaagca gagacccctg aagatacgga tgttagacgg aacggtgaag
      361 actatcatgg tggatgactc caagaccgtc acagacatgc tcatgaccat ttgtgcccga
      421 attggtatca ccaaccatga tgagtattca ctggttcgag agctgatgga agaaaagaaa
      481 gatgagggga cagggaccct gagaaaagac aagaccctac tgcgagatga aaagaagatg
      541 gagaagctaa agcagaagtt gcacacagac gatgagttga actggctgga ccatgggcgg
      601 acactgaggg aacagggagt ggaagagcat gagacgttgc tgctgcggag aaagttcttc
      661 tactcagacc agaatgtgga ttcccgagac cctgtacagc tgaaccttct ctatgtgcag
      721 gcacgagatg acatcctgaa tggctcccat cctgtctcct ttgacaaggc ctgtgaattt
      781 gcaggcttcc agtgccagat ccagtttgga cctcacaatg aacagaagca caaggctggc
      841 ttccttgacc tgaaggactt cctgcccaag gagtatgtga agcagaaggg agagcgtaag
      901 atctttcagg cacacaagaa ttgtgggcag atgagtgaaa ttgaggccaa ggtacgctac
      961 gtgaagctgg cccgttccct caagacttac ggtgtctcct tcttcctagt caaggaaaag
     1021 atgaagggga agaataaact ggtgcccagg ctgttgggca tcactaagga gtgtgtgatg
     1081 cgtgtggatg agaagaccaa ggaggtgatt caggaatgga gcctcaccaa catcaaacgc
     1141 tgggctgcct ctcccaagag ctttactctg gactttggag actatcagga tggctactac
     1201 tcagtacaga cgacagaagg cgagcagatc gcacagctca ttgctggcta catagatatc
     1261 atccttaaga agaaaaaaag caaggaccat tttgggctgg agggagatga agagtctact
     1321 atgttggagg actcagtgtc tcccaaaaag tcaacagtcc ttcagcagca gtacaaccga
     1381 gtggggaaag tggagcacgg ctctgtggct ctgccagcca tcatgcgctc tggagcctct
     1441 ggtcctgaga atttccaggt ggggagcatg ccacctgccc agcagcagat caccagtggc
     1501 cagatgcacc gaggacacat gccacctctg acttcagccc agcaggcgct cactggaacc
     1561 attaactcca gcatgcaggc tgtgcaggct gcccaggcca ctctggatga ctttgagact
     1621 ctaccccctc ttggccagga tgctgcctcc aaagcttggc gtaagaacaa gatggatgaa
     1681 tcaaagcatg agatccactc ccaggtagat gccatcacag ctggcactgc ctctgtggta
     1741 aacctgacag caggagatcc tgcagagaca gactatacag cagtgggctg tgcagtcacc
     1801 accatctctt ccaacctgac ggagatgtcc cgtggggtga agctattggc tgccctgctg
     1861 gaggatgaag gcggcaatgg ccggcccctt ctgcaagcag caaagggcct tgcaggggct
     1921 gtgtcggaac tgcttcgcag tgctcagccc gccagcgctg agcctcgtca gaacctgctg
     1981 caggcagccg ggaacgtggg ccaggccagt ggggagctgt tgcagcaaat tggggaaagt
     2041 gacactgacc cccacttcca ggatgttcta atgcagctag caaacgcagt ggcaagtgct
     2101 gcagccgccc tggtcctcaa ggccaagagt gtggcccagc gcacagagga ttccgggctt
     2161 cagacccaag tgattgctgc agctacgcaa tgtgctctgt ccacttccca gctggtggcc
     2221 tgtaccaagg tggtagcacc tacaatcagc tcacctgtgt gccaagaaca gctagtcgag
     2281 gctgggcgac tggtggccaa agctgtggag ggctgtgtgt ctgcctcgca agcagctaca
     2341 gaagatggac agctgcttcg aggggtagga gcagcagcca cggctgtcac ccaggccctc
     2401 aatgagctgc tgcagcacgt gaaggcccac gccaccggag ctgggcctgc cggtcggtat
     2461 gaccaagcca ctgataccat cctcaccgtc actgaaaaca tcttcagctc catgggtgat
     2521 gctggggaga tggtgcgcca ggcccgcatc ctggcccaag ccacctcaga cctggtcaat
     2581 gctatcaagg ccgatgctga gggggagagt gatctggaga actctagaaa gctcctgagt
     2641 gctgccaaga tcctcgctga tgccaccgcc aagatggtgg aggcggccaa gggagcagcc
     2701 gcccaccctg acagtgagga acagcagcag cgactgcgtg aagcagctga ggggcttcgc
     2761 atggccacca atgcagctgc gcagaacgcc atcaagaaga agttggtgca gcgcctggag
     2821 catgcagcca agcaagctgc agcctctgca acacagacca ttgctgcagc ccaacatgca
     2881 gcctctgccc ccaaggcctc tgcgggcccc cagcccctac tggtgcagag ctgtaaggcc
     2941 gtggcagagc agattccgtt gctggtgcag ggtgtccgag gaagtcaagc tcagcctgac
     3001 agccctagtg ctcagctcgc cctcattgct gccagccaga gcttcctgca gccaggtggg
     3061 aagatggtgg cagcggcaaa agcctcagtg ccaacaattc aggaccaggc ttcagcgatg
     3121 cagctgagtc agtgcgcaaa gaacctaggc actgccctgg ccgagctgcg cactgctgct
     3181 cagaaggctc aggaggcatg tggacctttg gagatggatt ctgcactgag tgttgtacaa
     3241 aatctagaga aagacctaca ggaaataaaa gcagcagctc gagatggcaa gcttaagccc
     3301 ttacctgggg aaacaatgga gaagtgtacc caagatcttg gcaacagcac caaagcggtg
     3361 agctctgcca tcgccaagct gctgggggag attgcccagg gcaacgagaa ctatgcaggt
     3421 attgcagctc gggatgtagc tggtggacta aggtcactag cccaggctgc gcggggtgtg
     3481 gctgcgctga catcagatcc tgcagtgcag gccattgtgc ttgacacagc cagcgacgtt
     3541 ctggacaagg ccagcagcct cattgaagag gcaaaaaaag catctggaca cccaggggac
     3601 ccagaaagcc agcaaaggct tgctcaggta gctaaagcag tgacccaagc cctgaaccgc
     3661 tgtgtcagct gcctgcctgg ccaaagagac gtggataatg ccctacgagc cgttggagat
     3721 gccagcaagc gactcctgag tgacttgctt cctccaagca cggggacatt tcaagaagca
     3781 cagagccgat tgaatgaagc tgcagctggg ttaaatcagg ccgccacaga actggtgcaa
     3841 gcctcccgag gaactcctca ggacctggct cgggcctccg gccgatttgg acaggacttc
     3901 agcaccttcc tggaagctgg tgtggagatg gccggacagg ccccgagcca ggaggaccga
     3961 gcccaggtgg tgtccaacct gaagggcata tccatgtctt caagcaaact tctccttgcc
     4021 gctaaggcct tgtccacaga ccctgcttct cccaacctca agagtcagct ggctgcggct
     4081 gcccgggcag tgacggacag catcaaccag ctcatcacca tgtgcaccca gcaggcacct
     4141 ggccagaagg agtgtgacaa tgcacttcgg cagctagaga cagtccgaga actcctggag
     4201 aatccagtcc agcccatcaa cgacatgtcc tacttcggtt gcttggacag tgtcatggag
     4261 aactccaagg tcctaggtga ggccatgact ggcatctccc aaaatgccaa gaatggaaat
     4321 ctgccggagt ttggagatgc cattgccaca gcctccaagg ctctctgtgg cttcacggag
     4381 gcagccgcac aggcagcata tctagttggt gtctctgacc ccaacagcca agctggacag
     4441 caaggactgg tggaacccac acagtttgcc cgtgcaaacc aggcaattca gatggcctgt
     4501 cagagtctgg gggagcctgg ctgtacccag gcccaggtgt tatctgcagc cactattgta
     4561 gccaaacaca catctgcatt gtgtaacagc tgtcgcctgg cttccgctag gactgccaat
     4621 cccactgcca agcgccagtt tgtacagtca gccaaggagg tggccaacag tacagccaat
     4681 cttgtcaaga ccatcaaggc actagatggg gacttcacag aagagaaccg tgcccagtgc
     4741 cgagcagcca cagcccctct gctggaagct gtggataacc tgagtgcctt tgcctccaac
     4801 cctgagttct ccagcgtccc tgcccagatc agccctgagg gcagagcagc catggagccc
     4861 attgtaatct ctgctaagac aatgttggag agtgctggag ggctcatcca gacagctcgg
     4921 gcgttagcag tcaatccccg agacccccca cgttggtctg tgctagctgg ccactctcgg
     4981 actgtctcag attccatcaa gaaacttatt acaagcatga gggacaaagc cccagggcag
     5041 ctggagtgtg agacagccat tgcggctctg aacagctgct tgcgggacct agatcaggct
     5101 tcgcttgccg ctgtcagcca gcagcttgct ccccgtgaag gaatctctca agaggctttg
     5161 cacacccaga tgctcactgc agtgcaagaa atttctcatc tcattgagcc gctggccagc
     5221 gctgctcggg ctgaagcctc ccagctggga cacaaggtgt cccaaatggc ccagtacttt
     5281 gaaccactca ccctggctgc agtgggtgct gcgtctaaga ccctgagcca cccgcagcag
     5341 atggcacttc tggaccagac taaaacgttg gcagagtctg ccttgcagtt gctatatact
     5401 gccaaggagg ctggtggtaa ccccaagcaa gcagcacaca cccaggaagc cctggaggag
     5461 gctgtgcaga tgatgacaga ggccgtagaa gacctgacga cgaccctcaa cgaagcagcc
     5521 agtgctgcag gagtcgttgg cggcatggtg gactctatca ctcaggccat caaccagcta
     5581 gatgaaggac ctatgggtga cccagaaggc tcattcgtag attaccagac aaccatggtg
     5641 aggacagcca aggccattgc tgtcactgtt caggagatgg taaccaagtc aaacaccagc
     5701 cctgaagagc taggccctct tgccaaccag ctgaccagtg actatggccg actggcctca
     5761 caagccaagc ctgcagctgt ggctgctgaa aatgaagaga taggcgctca catcaaacac
     5821 cgagtacagg agctgggcca tggctgctct gctctggtca ccaaggcagg tgccttgcag
     5881 tgtagcccca gtgatgtcta caccaagaag gagctcatag agtgtgcccg cagagtgtca
     5941 gagaaggtct cccatgttct ggctgcactc caggctggga atcgtggtac ccaggcctgc
     6001 attacagcag ccagtgctgt gtctggtatc attgctgacc tcgacaccac catcatgttc
     6061 gctactgctg gcacacttaa ccgtgagggt gctgaaactt ttgctgacca ccgggagggt
     6121 atcttaaaga cagcaaaggt tcttgtggag gacaccaagg tcctagtgca gaatgcagct
     6181 gggagccagg agaagttggc acaagccgcc cagtcctccg tggccaccat tacccgcctc
     6241 gctgatgtgg tcaagctcgg tgcagccagc ctaggagccg aagaccctga aactcaggtg
     6301 gtgctgatca atgcagtaaa ggacgtagcc aaggccctgg gtgacctcat cagcgctacg
     6361 aaggctgcag cgggcaaagt tggggatgac cctgcagtgt ggcagctcaa gaactctgcc
     6421 aaggtgatgg tgaccaatgt gacatcattg ctcaagacag tgaaggctgt ggaagatgag
     6481 gccaccaaag gcacacgggc cctagaggca accacagagc acatacgtca ggaactggcg
     6541 gtcttctgtt ccccagagcc acctgccaag acctctaccc ctgaagactt catccgaatg
     6601 accaagggta ttactatggc aacagccaaa gccgttgctg ctggcaattc ctgtcgacag
     6661 gaagatgtca ttgccacagc caatctgagc cgacgggcta ttgcggacat gcttcgggct
     6721 tgcaaggaag cagctttcca cccagaagtg gcacctgatg tacggctccg agccctgcac
     6781 tatggccggg agtgtgccaa tggttacctg gaactgctgg accacgtgct gctgaccctt
     6841 cagaagccaa acccagacct gaagcagcag ctaactggac actcaaagcg ggttgctggc
     6901 tctgtgactg agctcatcca ggctgctgaa gccatgaaag gaacagagtg ggtggaccca
     6961 gaggacccta ctgtcattgc tgagaatgag ctcttgggag ccgcagccgc catcgaggct
     7021 gcagccaaga agctggagca gctgaagccc agggccaaac ccaaggaggc ggatgagtcc
     7081 ttgaactttg aggaacaaat cctagaagct gccaagtcca tcgctgcagc caccagtgca
     7141 ctggtaaagg ctgcgtcagc agcccagagg gagctggtgg ctcaaggaaa ggtgggcgcc
     7201 attccagcca atgcactgga tgatgggcag tggtcgcagg gcctcatttc cgctgcccgt
     7261 atggtggctg cggccaccaa caatctgtgt gaagcagcta acgcagctgt ccagggccat
     7321 gctagccagg agaaactcat ttcctcagcc aagcaagtag ctgcctccac agctcagctc
     7381 ctggtagctt gcaaggtcaa agcagatcag gactctgagg caatgaaacg gctccaggct
     7441 gctggcaatg cagtgaagag ggcctcagat aacctggtaa aggcggccca gaaagctgca
     7501 gccttcgaag accaggagaa tgagacggtg gtggtgaagg agaaaatggt tgggggcatt
     7561 gcccagatta tcgcagcaca ggaagagatg cttcggaagg aacgagagct ggaagaggct
     7621 cggaaaaagc tcgcccagat ccggcagcag cagtacaagt tcttgccttc agagcttcga
     7681 gacgagcgcg gctccgcgtc ccgatcaccg gtcgccacca tggtgagcaa gggcgaggag
     7741 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     7801 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
     7861 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
     7921 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     7981 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     8041 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     8101 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     8161 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     8221 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
     8281 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag
     8341 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     8401 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     8461 taatcagcca taccacat
//
