LOCUS       sequence-87240-e        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane , Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-UbiquitinC-N-18
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..263
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     regulatory      312..321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(318..320,321..323,324..1037)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(363..384)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(624..643)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     971..992
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gccatgctag tgacgtcaga tccgctagcg ccaccatgca gatcttcgtg aagactctga
       61 ctggtaagac catcaccctc gaggttgagc ccagtgacac cattgagaat gtcaaggcaa
      121 agatccaaga taaggaaggc atccctcctg accagcagag gctgatcttt gctggaaaac
      181 agctggaaga tgggcgcacc ctgtctgact acaacatcca gaaagagtcc accctgcacc
      241 tggtactccg tctcagaggt gggggtggaa ttctgcagtc gacggtaccg cgggcccggg
      301 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      361 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      421 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
      481 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct
      541 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      601 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      661 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      721 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
      781 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
      841 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      901 tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga
      961 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1021 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacattgta
     1081 gagtacct
//
