LOCUS       sequence-87242-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 529, Emission: 539.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZsYellow1-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      210..219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             216..911
                     /codon_start=1
                     /product="Zoanthus yellow fluorescent protein"
                     /label=ZsYellow1
                     /note="mammalian codon-optimized"
                     /translation="MAHSKHGLKEEMTMKYHMEGCVNGHKFVITGEGIGYPFKGKQTIN
                     LCVIEGGPLPFSEDILSAGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWGRSFLFEDGAV
                     CICNVDITVSVKENCIYHKSIFNGVNFPADGPVMKKMTTNWEASCEKIMPVPKQGILKG
                     DVSMYLLLKDGGRYRCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKWQLTEH
                     AIAFPSALA"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctggtacc ccttgcagct tccagttgtc
       61 aggaagagaa ggctcccatg gatcctgtgg tggtgctggg gctctgtctc tcctgtttgc
      121 ttctcctttc actctggaaa cagagctatg ggggagggaa gcttcgaatt ctgcagtcga
      181 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggc ccacagcaag cacggcctga
      241 aggaggagat gaccatgaag taccacatgg agggctgcgt gaacggccac aagttcgtga
      301 tcaccggcga gggcatcggc taccccttca agggcaagca gaccatcaac ctgtgcgtga
      361 tcgagggcgg ccccctgccc ttcagcgagg acatcctgag cgccggcttc aagtacggcg
      421 accggatctt caccgagtac ccccaggaca tcgtggacta cttcaagaac agctgccccg
      481 ccggctacac ctggggccgg agcttcctgt tcgaggacgg cgccgtgtgc atctgtaacg
      541 tggacatcac cgtgagcgtg aaggagaact gcatctacca caagagcatc ttcaacggcg
      601 tgaacttccc cgccgacggc cccgtgatga agaagatgac caccaactgg gaggccagct
      661 gcgagaagat catgcccgtg cctaagcagg gcatcctgaa gggcgacgtg agcatgtacc
      721 tgctgctgaa ggacggcggc cggtaccggt gccagttcga caccgtgtac aaggccaaga
      781 gcgtgcccag caagatgccc gagtggcact tcatccagca caagctgctg cgggaggacc
      841 ggagcgacgc caagaaccag aagtggcagc tgaccgagca cgccatcgcc ttccccagcg
      901 ccctggcctg aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
      961 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     1021 aat
//
