LOCUS       sequence-87250-e        2609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1851..1860
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1857..2570
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agaccgccat ggaagggaag tggttgctgt
       61 gtatgttact ggtgcttgga actgctattg ttgaggctca tgatggacat gatgatgatg
      121 tgattgatat tgaggatgac cttgacgatg tcattgaaga ggtagaagac tcaaaaccag
      181 ataccactgc tcctccttca tctcccaagg ttacttacaa agctccagtt ccaacagggg
      241 aagtatattt tgctgattct tttgacagag gaactctgtc agggtggatt ttatccaaag
      301 ccaagaaaga cgataccgat gatgaaattg ccaaatatga tggaaagtgg gaggtagagg
      361 aaatgaagga gtcaaagctt ccaggtgata aaggacttgt gttgatgtct cgggccaagc
      421 atcatgccat ctctgctaaa ctgaacaagc ccttcctgtt tgacaccaag cctctcattg
      481 ttcagtatga ggttaatttc caaaatggaa tagaatgtgg tggtgcctat gtgaaactgc
      541 tttctaaaac accagaactc aacctggatc agttccatga caagacccct tatacgatta
      601 tgtttggtcc agataaatgt ggagaggact ataaactgca cttcatcttc cgacacaaaa
      661 accccaaaac gggtatctat gaagaaaaac atgctaagag gccagatgca gatctgaaga
      721 cctattttac tgataagaaa acacatcttt acacactaat cttgaatcca gataatagtt
      781 ttgaaatact ggttgaccaa tctgtggtga atagtggaaa tctgctcaat gacatgactc
      841 ctcctgtaaa tccttcacgt gaaattgagg acccagaaga ccggaagccc gaggattggg
      901 atgaaagacc aaaaatccca gatccagaag ctgtcaagcc agatgactgg gatgaagatg
      961 cccctgctaa gattccagat gaagaggcca caaaacccga aggctggtta gatgatgagc
     1021 ctgagtacgt acctgatcca gacgcagaga aacctgagga ttgggatgaa gacatggatg
     1081 gagaatggga ggctcctcag attgccaacc ctagatgtga gtcagctcct ggatgtggtg
     1141 tctggcagcg acctgtgatt gacaacccca attataaagg caaatggaag cctcctatga
     1201 ttgacaatcc cagttaccag ggaatctgga aacccaggaa aataccaaat ccagatttct
     1261 ttgaagatct ggaacctttc agaatgactc cttttagtgc tattggtttg gagctgtggt
     1321 ccatgacctc tgacattttt tttgacaact ttatcatttg tgctgatcga agaatagttg
     1381 atgattgggc caatgatgga tggggcctga agaaagctgc tgatggggct gctgagccag
     1441 gcgttgtggg gcagatgaac gaggcagctg aagagcgccc gtggctgtgg gtagtctata
     1501 ttctaactgt agcccttcct gtgttcctgg ttatcctctt ctgctgttct ggaaagaaac
     1561 agaccagtgg tatggagtat aagaaaactg atgcacctca accggatgtg aaggaagagg
     1621 aagaagagaa ggaagaggaa aaggacaagg gagatgagga ggaggaagga gaagagaaac
     1681 ttgaagagaa acagaaaagt gatgctgaag aagatggtgg cactgtcagt caagaggagg
     1741 aagacagaaa acctaaagca gaggaggatg aaattttgaa cagatcacca agaaacagaa
     1801 agccacgaag agaggggggt gcgtcaggcg gcagcggatc tggaccggtc gccaccatgg
     1861 tgagcaaggg cgaggggcaa tcgaagcatg gactaaagga ggagatgacg gtgaagtacc
     1921 acatggaagg ttgcgttaat ggtcacaaat ttgtcattac tggagagggc attggaaacc
     1981 cttttaaggg taagcaaacc gcaaatttgt gtgtgataga aggaggcccg ctgccgttct
     2041 cggaggacat tttaagtccg ggttttaaat atggtgaccg gattttcaca gagtacccac
     2101 aggatattgt agattacttc aagaactcat gtccagcggg ctatacgtgg gaaaggagct
     2161 acctctttga ggacggagcg gtctgtcgat gcaacgtgga cataacagtc tctgaaaagg
     2221 agaactgcat ctatcacaaa agtatcttca gaggggtgaa ttttcccgcc gacggccccg
     2281 taatgaagaa aatgaccact aattgggaag ctagtaccga gaaaattgtg cctgttccaa
     2341 agcaagggat attaaaggga aaggtcaaaa tgtgcctgtt gctgaaggat ggcggtcgtt
     2401 atcattgcca gtttgatacg gtatataaag ctaaatcagt gccctccaag atgccagaat
     2461 ggcattttat acagcataag ctccttaggg aggaccgctc tgatgctaaa aaccagaaat
     2521 ggcaattaac agaacatgca atcgcaggca tggacgagct gtacaagtaa gcggccgcga
     2581 ctctagatca taatcagcca taccacatg
//
