LOCUS       sequence-87251-e        1343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    530..582
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      590..599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             596..1309
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcca ccatgggccc caagcggcgg cagctgacgt tccgggagaa
       61 gtcgcggatc atccaggagg tggaggagaa cccggaccta cgcaagggcg agatcgcgcg
      121 gcgcttcaac atcccgccgt ccacgctgag cacgatcctg aagaacaagc gcgccatcct
      181 ggcgtcggag cgcaagtacg gggtggcctc cacctgccgc aagaccaaca agctgtctcc
      241 ctacgacaag ctcgagggct tgctcatcgc ctggttccag cagatccgcg ccgccggcct
      301 gccggtcaag ggcatcatcc tcaaggagaa ggcgctgcgc atagccgagg agctgggcat
      361 ggacgacttc accgcctcca acggctggct ggaccgcttc cgccggcgcc acggcgtggt
      421 gtcctgcagc ggcgtggccc gcgcccgcgc gcgaaacgct gccccccgca ccccggcggc
      481 gcctgccagt ccggccgcgg tgccctcgga gggcagtggc gggagcactc tcgagctcaa
      541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt
      601 gagcaagggc gaggggcaat cgaagcatgg actaaaggag gagatgacgg tgaagtacca
      661 catggaaggt tgcgttaatg gtcacaaatt tgtcattact ggagagggca ttggaaaccc
      721 ttttaagggt aagcaaaccg caaatttgtg tgtgatagaa ggaggcccgc tgccgttctc
      781 ggaggacatt ttaagtccgg gttttaaata tggtgaccgg attttcacag agtacccaca
      841 ggatattgta gattacttca agaactcatg tccagcgggc tatacgtggg aaaggagcta
      901 cctctttgag gacggagcgg tctgtcgatg caacgtggac ataacagtct ctgaaaagga
      961 gaactgcatc tatcacaaaa gtatcttcag aggggtgaat tttcccgccg acggccccgt
     1021 aatgaagaaa atgaccacta attgggaagc tagtaccgag aaaattgtgc ctgttccaaa
     1081 gcaagggata ttaaagggaa aggtcaaaat gtgcctgttg ctgaaggatg gcggtcgtta
     1141 tcattgccag tttgatacgg tatataaagc taaatcagtg ccctccaaga tgccagaatg
     1201 gcattttata cagcataagc tccttaggga ggaccgctct gatgctaaaa accagaaatg
     1261 gcaattaaca gaacatgcaa tcgcaggcat ggacgagctg tacaagtaag cggccgcgac
     1321 tctagatcat aatcagccat acc
//
