LOCUS       sequence-87253-e        2250 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytokeratin, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Keratin14-N-18
FEATURES             Location/Qualifiers
     source          1..2250
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1493..1502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1499..2212
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 aggtgacgtc agatccgcta gcgccaccat gactacctgc agccgccagt tcacctcctc
       61 cagctccatg aagggctcct gcggcatcgg gggcggcatc gggggcggct ccagccgcat
      121 ctcctccgtc ctggccggag ggtcctgccg cgcccccagc acctacgggg gcggcctgtc
      181 tgtctcatcc tcccgcttct cctctggggg agcctatggg ttggggggcg gctatggcgg
      241 tggcttcagc agcagcagca gcagctttgg tagtggcttt gggggaggat atggtggtgg
      301 ccttggtgct ggcttgggtg gtggctttgg tggtggcttt gctggtggtg atgggcttct
      361 ggtgggcagt gagaaggtga ccatgcagaa cctcaacgac cgcctggcct cctacctgga
      421 caaggtgcgt gctctggagg aggccaacgc cgacctggaa gtgaagatcc gtgactggta
      481 ccagaggcag cggcctgctg agatcaaaga ctacagtccc tacttcaaga ccattgagga
      541 cctgaggaac aagattctca cagccacagt ggacaatgcc aatgtccttc tgcagattga
      601 caatgcccgt ctggccgcgg atgacttccg caccaagtat gagacagagt tgaacctgcg
      661 catgagtgtg gaagccgaca tcaatggcct gcgcagggtg ctggacgaac tgaccctggc
      721 cagagctgac ctggagatgc agattgagag cctgaaggag gagctggcct acctgaagaa
      781 gaaccacgag gaggagatga atgccctgag aggccaggtg ggtggagatg tcaatgtgga
      841 gatggacgct gcacctggcg tggacctgag ccgcattctg aacgagatgc gtgaccagta
      901 tgagaagatg gcagagaaga accgcaagga tgccgaggaa tggttcttca ccaagacaga
      961 ggagctgaac cgcgaggtgg ccaccaacag cgagctggtg cagagcggca agagcgagat
     1021 ctcggagctc cggcgcacca tgcagaacct ggagattgag ctgcagtccc agctcagcat
     1081 gaaagcatcc ctggagaaca gcctggagga gaccaaaggt cgctactgca tgcagctggc
     1141 ccagatccag gagatgattg gcagcgtgga ggagcagctg gcccagctcc gctgcgagat
     1201 ggagcagcag aaccaggagt acaagatcct gctggacgtg aagacgcggc tggagcagga
     1261 gatcgccacc taccgccgcc tgctggaggg cgaggacgcc cacctctcct cctcccagtt
     1321 ctcctctgga tcgcagtcat ccagagatgt gacctcctcc agccgccaaa tccgcaccaa
     1381 ggtcatggat gtgcacgatg gcaaggtggt gtccacccac gagcaggtcc ttcgcaccaa
     1441 gaacagcagc ggtggagcaa gcgcagccag tggtagcgcg gatccaccgg tcgccaccat
     1501 ggtgagcaag ggcgaggggc aatcgaagca tggactaaag gaggagatga cggtgaagta
     1561 ccacatggaa ggttgcgtta atggtcacaa atttgtcatt actggagagg gcattggaaa
     1621 cccttttaag ggtaagcaaa ccgcaaattt gtgtgtgata gaaggaggcc cgctgccgtt
     1681 ctcggaggac attttaagtc cgggttttaa atatggtgac cggattttca cagagtaccc
     1741 acaggatatt gtagattact tcaagaactc atgtccagcg ggctatacgt gggaaaggag
     1801 ctacctcttt gaggacggag cggtctgtcg atgcaacgtg gacataacag tctctgaaaa
     1861 ggagaactgc atctatcaca aaagtatctt cagaggggtg aattttcccg ccgacggccc
     1921 cgtaatgaag aaaatgacca ctaattggga agctagtacc gagaaaattg tgcctgttcc
     1981 aaagcaaggg atattaaagg gaaaggtcaa aatgtgcctg ttgctgaagg atggcggtcg
     2041 ttatcattgc cagtttgata cggtatataa agctaaatca gtgccctcca agatgccaga
     2101 atggcatttt atacagcata agctccttag ggaggaccgc tctgatgcta aaaaccagaa
     2161 atggcaatta acagaacatg caatcgcagg catggacgag ctgtacaagt aagcggccgc
     2221 gactctagat cataatcagc cataccacat
//
