LOCUS       sequence-87254-e        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      396..405
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             402..1115
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 accatgctag tgacgtcaga tccgctagcg ccaccatgaa gttaagtcgc cagttcaccg
       61 tgtttggcag cgcgatcttc tgcgtcgtaa tcttctcact ctacctgatg ctggacaggg
      121 gtcacttgga ctaccctcgg ggcccgcgcc aggagggctc ctttccgcag ggccagcttt
      181 caatattgca agaaaagatt gaccatttgg agcgtttgct cgctgagaac aacgagatca
      241 tctcaaatat cagagactca gtcatcaacc tgagcgagtc tgtggaggac ggcccgcggg
      301 ggtcaccagg caacgccagc caaggctcca tccacctcca ctcgccacag ttggccctgc
      361 aggctgaccc cagctccgga tcggatccac cggtcgccac catggtgagc aagggcgagg
      421 ggcaatcgaa gcatggacta aaggaggaga tgacggtgaa gtaccacatg gaaggttgcg
      481 ttaatggtca caaatttgtc attactggag agggcattgg aaaccctttt aagggtaagc
      541 aaaccgcaaa tttgtgtgtg atagaaggag gcccgctgcc gttctcggag gacattttaa
      601 gtccgggttt taaatatggt gaccggattt tcacagagta cccacaggat attgtagatt
      661 acttcaagaa ctcatgtcca gcgggctata cgtgggaaag gagctacctc tttgaggacg
      721 gagcggtctg tcgatgcaac gtggacataa cagtctctga aaaggagaac tgcatctatc
      781 acaaaagtat cttcagaggg gtgaattttc ccgccgacgg ccccgtaatg aagaaaatga
      841 ccactaattg ggaagctagt accgagaaaa ttgtgcctgt tccaaagcaa gggatattaa
      901 agggaaaggt caaaatgtgc ctgttgctga aggatggcgg tcgttatcat tgccagtttg
      961 atacggtata taaagctaaa tcagtgccct ccaagatgcc agaatggcat tttatacagc
     1021 ataagctcct tagggaggac cgctctgatg ctaaaaacca gaaatggcaa ttaacagaac
     1081 atgcaatcgc aggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
     1141 agccatacca catttgtaga gtagcct
//
