LOCUS       sequence-87258-e        3546 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 488, 
            Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3546)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP-CAF1-C-6
FEATURES             Location/Qualifiers
     source          1..3546
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2556..2567
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      241 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      481 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagtccg gactcagatc ccatatgttg gaggagccgg
      781 aggccgcgac gcggacagcc gcggccgtgg attgcaaaga cagacccggg ttcccagtga
      841 agaggttaat acaagcccgc ttgcccttca aacgcctgaa ccttgttccc aaggagaaag
      901 ttgaggaaga cacatcacca aaagcagctg tggaaagcaa agtcccggat ctacaattgt
      961 ctttgggcac ttttgaaagc cagtgtcaca caggctctca cgtaggcttg agtacaaagt
     1021 tggtcggtgg ccagggcccc attgatagct tcttgagggc aacaatcaag ccagtaccga
     1081 gtgtggtcat tatcgacctg acggagaact gcagtgacat tccagacagc cctgagggcc
     1141 acagtgaact aagtcctgac actgctggtg tggtcaccac agtggaagga gctgccaagc
     1201 agcaggagca ctctgcagca gagctgtgtc tcctagagac cccttcagac atcacttgtc
     1261 acatggaaga ggagcctggg agtccaggag acccaaagag gacgggtgac tgtcaggctg
     1321 gttcactaca gagctgtccg gagctgaccc caggttctag gacatgcccc accaaggagt
     1381 tatcaagctg gagcaaggca ggggatctcc tatttataga gaaagtgcct gtggtggtgc
     1441 tagaggacat cctggccact aagccctcca ttgcctctct tcccatgatg tccctggata
     1501 ggagtgtcac ttcagagagt gaaatactgg agtcctgccc cgaggatgac tccatactga
     1561 gccactcctc caccaactcc tcttctccaa ccagctctcc tgagggacca tccacacccc
     1621 cagaacatcg gggtgggaga agctccccct ccaccccagc ctgcagagtt gccaagaact
     1681 ttgtcaaagg ctccacagag aagggcagga gcaaactgca cagagataga gaacagcaga
     1741 gggaagagaa ggaaaagctg agggaagaga tacgacgagc caaggaggag gcacggaaga
     1801 agaaggagga ggagaaggag ctgaaggaaa aggagcggag ggaaaagcgg gagaaagatg
     1861 agaaggagaa ggcggagaag cagcggctaa aggaggaaaa acgcaaggag cggcaggaag
     1921 ctctggaggc taaactggag gagaagagga agaaggagga agagaagcgg ttacgggagg
     1981 aagagaagcg gttacgggaa gaagagaagc gcattaaggc agaaaaggca gagatcactc
     2041 gattcttcca gaaaccgaag actccgcagg ctcccaagac cctggccggt tcctgtggta
     2101 agtttgcccc ctttgaaatc aaagaacaca tggtgctggc tccacggtgc cgagctgcct
     2161 tggatcaaga cctgtgcgat cagctggacc agctcctgca gcagcagagt gtggcaagca
     2221 ccttcctcag tgacctaaaa agccggctgc ccttgagatc ggggcccacc cgggtctgtg
     2281 gccatgacac agacatcatg aacagggatg tggtcattgt ggagagcagc aaggtggacg
     2341 gtgtgtctga aaggaagaag tttggccgca tgaagcttct gcagttctca gagaaccacc
     2401 ggccagcata ctgggggacg tggaacaaga agacagccat catccgcccc cggaacccct
     2461 gggcccagga caaagacctt cttgactatg aggtagacag tgacgatgag tgggaagagg
     2521 aggaacctgg ggagtctttg tcccacagtg agggggatga ggatgacgat gtgggggaag
     2581 atgaggatga ggacgatggc ttctttgtgc cccatgggta cttatctgaa gatgaaggcg
     2641 tgactgagga gtgtgcagac cctgagaacc acaaagtaca ccagaagctg aaggccaagg
     2701 agtgggatga gctcctagcc aagggcaagc gctttcgtgt gctgcagcct gtgcacgtgg
     2761 gatgtgtatg ggcatcagag gcagccaact gcacgagttc cgacttgaag ctgctgcagc
     2821 agttcactgc atgcctgctg gatgtggcat cccctgatga gccagaaccc ggggcctcca
     2881 ggagggaaaa gagggaccag catatcctgg cccagttgct tccgctgcta catggcaatg
     2941 tgaatgggag caaggtgatc atccatgaat tccaggagca atgccgcagg ggcctgctga
     3001 cccttccaag tcccacccca cacctgcaga tgcccaactt ggaggatgct gttgctgtgc
     3061 catccaaggc caggctgaag cgccttatct ctgagaactc tgcctatgag aagcggccca
     3121 acttccgtat gtgctggtat gtgcacccag aggtgctcaa gagctttggc caggagtgcc
     3181 tgccagtgcc ctgccagtgg acctacatca ccaccatgcc ctcagcaccc agggaagaca
     3241 gtggcagtgc ttctactgag gggcctggcc agagcacacc tatgctactc aagcggaagc
     3301 cagcggccac catgtgcatc acgcagttta tgaagaagcg gcgctatgat ggacaggttg
     3361 ggtctgggga catggatggc ttccaggcag acacagaaga ggatgaagag gatgacacag
     3421 actgtatgat catagatgtg ccagatgttg ggagtgacgt gtcagaagct cccatacctg
     3481 ctcccacact ttgcaagtga gatccaccgg atctagataa ctgatcataa tcagccatac
     3541 cacatt
//
