LOCUS       sequence-87262-e        1365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1365)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gcgcggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      241 gtgcttcagc cgctaccccg accacatgaa gcgccacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatcag cttcaaggac gacggcacct acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
      481 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      541 caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg tgctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactcacggc atggacgagc tgtacaagtc cggactcaga tctggcagca gtggaggttc
      781 tgggggcaaa tacgtagact cggagggaca tctctacacc gttcccatcc gggaacaggg
      841 caacatctac aagcccaaca acaaggccat ggcagacgag ctgagcgaga agcaagtgta
      901 cgacgcgcac accaaggaga tcgacctggt caaccgcgac cctaaacacc tcaacgatga
      961 cgtggtcaag attgactttg aagatgtgat tgcagaacca gaagggacac acagttttga
     1021 cggcatttgg aaggccagct tcaccacctt cactgtgacg aaatactggt tttaccgctt
     1081 gctgtctgcc ctctttggca tcccgatggc actcatctgg ggcatttact tcgccattct
     1141 ctctttcctg cacatctggg cagttgtacc atgcattaag agcttcctga ttgagattca
     1201 gtgcatcagc cgtgtctatt ccatctacgt ccacaccgtc tgtgacccac tctttgaagc
     1261 tgttgggaaa atattcagca atgtccgcat caacttgcag aaagaaatat aagaattctg
     1321 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataac
//
