LOCUS       sequence-87264-e        1983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..757
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(86..107)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     694..715
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gttagtgacg tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga
       61 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      121 gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt
      181 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
      241 cggcgtggcc tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      301 cgccatgccc gaaggctacg tccaggagcg caccatctct ttcaaggacg acggtaccta
      361 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      421 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa
      481 cagccacaac gtctatatca cggccgacaa gcagaagaac ggcatcaagg ctaacttcaa
      541 gatccgccac aacgttgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
      601 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcc atcagtccgc
      661 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      721 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctggcagcgg
      781 tggaggcagc gcatccggcg gaagcggaag cgatgatgat atcgccgcgc tcgtcgtcga
      841 caacggctcc ggcatgtgca aggccggctt cgcgggcgac gatgcccccc gggccgtctt
      901 cccctccatc gtggggcgcc ccaggcacca gggcgtgatg gtgggcatgg gtcagaagga
      961 ttcctatgtg ggcgacgagg cccagagcaa gagaggcatc ctcaccctga agtaccccat
     1021 cgagcacggc atcgtcacca actgggacga catggagaaa atctggcacc acaccttcta
     1081 caatgagctg cgtgtggctc ccgaggagca ccccgtgctg ctgaccgagg cccccctgaa
     1141 ccccaaggcc aaccgcgaga agatgaccca gatcatgttt gagaccttca acaccccagc
     1201 catgtacgtt gctatccagg ctgtgctatc cctgtacgcc tctggccgta ccactggcat
     1261 cgtgatggac tccggtgacg gggtcaccca cactgtgccc atctacgagg ggtatgccct
     1321 cccccatgcc atcctgcgtc tggacctggc tggccgggac ctgactgact acctcatgaa
     1381 gatcctcacc gagcgcggct acagcttcac caccacggcc gagcgggaaa tcgtgcgtga
     1441 cattaaggag aagctgtgct acgtcgccct ggacttcgag caagagatgg ccacggctgc
     1501 ttccagctcc tccctggaga agagctacga gctgcctgac ggccaggtca tcaccattgg
     1561 caatgagcgg ttccgctgcc ctgaggcact cttccagcct tccttcctgg gcatggagtc
     1621 ctgtggcatc cacgaaacta ccttcaactc catcatgaag tgtgacgtgg acatccgcaa
     1681 agacctgtac gccaacacag tgctgtctgg cggcaccacc atgtaccctg gcattgccga
     1741 caggatgcag aaggagatca ctgccctggc acccagcaca atgaagatca agatcattgc
     1801 tcctcctgag cgcaagtact ccgtgtggat cggcggctcc atcctggcct cgctgtccac
     1861 cttccagcag atgtggatca gcaagcagga gtatgacgag tccggcccct ccatcgtcca
     1921 ccgcaaatgc ttctagggat ccaccggatc tagataactg atcataatca gccataccac
     1981 att
//
