LOCUS       sequence-87267-e        1860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-MAPTau-C-10
FEATURES             Location/Qualifiers
     source          1..1860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..750
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtccgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg
      241 gcctgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc tctttcaagg acgacggtac ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac
      481 aacgtctata tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc
      541 cacaacgttg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccatcagtc cgccctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggaagcg gcgggtccgg gagcggaagc
      781 gctgagcccc gccaggagtt cgaagtgatg gaagatcacg ctgggacgta cgggttgggg
      841 gacaggaaag atcagggggg ctacaccatg caccaagacc aagagggtga cacggacgct
      901 ggcctgaaag ctgaagaagc aggcattgga gacaccccca gcctggaaga cgaagctgct
      961 ggtcacgtga cccaagctcg catggtcagt aaaagcaaag acgggactgg aagcgatgac
     1021 aaaaaagcca agggggctga tggtaaaacg aagatcgcca caccgcgggg agcagcccct
     1081 ccaggccaga agggccaggc caacgccacc aggattccag caaaaacccc gcccgctcca
     1141 aagacaccac ccagctctgg tgaacctcca aaatcagggg atcgcagcgg ctacagcagc
     1201 cccggctccc caggcactcc cggcagccgc tcccgcaccc cgtcccttcc aaccccaccc
     1261 acccgggagc ccaagaaggt ggcagtggtc cgtactccac ccaagtcgcc gtcttccgcc
     1321 aagagccgcc tgcagacagc ccccgtgccc atgccagacc tgaagaatgt caagtccaag
     1381 atcggctcca ctgagaacct gaagcaccag ccgggaggcg ggaaggtgca aatagtctac
     1441 aaaccagttg acctgagcaa ggtgacctcc aagtgtggct cattaggcaa catccatcat
     1501 aaaccaggag gtggccaggt ggaagtaaaa tctgagaagc ttgacttcaa ggacagagtc
     1561 cagtcgaaga ttgggtccct ggacaatatc acccacgtcc ctggcggagg aaataaaaag
     1621 attgaaaccc acaagctgac cttccgcgag aacgccaaag ccaagacaga ccacggggcg
     1681 gagatcgtgt acaagtcgcc agtggtgtct ggggacacgt ctccacggca tctcagcaat
     1741 gtctcctcca ccggcagcat cgacatggta gactcgcccc agctcgccac gctagctgac
     1801 gaggtgtctg cctccctggc caagcagggt ttgtgaggat ccaccggatc tagataactg
//
