LOCUS       sequence-87268-e        6764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..756
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(85..106)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     693..714
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctagtgacgc tcgagccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag
       61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      121 ttcagcgtcc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc
      181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
      241 ggcgtggcct gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      301 gccatgcccg aaggctacgt ccaggagcgc accatctctt tcaaggacga cggtacctac
      361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac
      481 agccacaacg tctatatcac ggccgacaag cagaagaacg gcatcaaggc taacttcaag
      541 atccgccaca acgttgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcca tcagtccgcc
      661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      721 gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc tggcagcggt
      781 ggaggcagcg catccggcgg aagcggaagc gctcagcagg ctgcagacaa gtacctctat
      841 gtggataaaa acttcatcaa taacccgctg gcccaagctg actgggctgc caagaagttg
      901 gtatgggtgc cttccagcaa gaatggcttt gaaccagcca gcctcaagga ggaggtggga
      961 gaagaggcca ttgtagagct ggtagagaat gggaagaagg tgaaggtgaa caaggacgac
     1021 atccagaaga tgaacccacc caagttctcc aaggtggagg acatggcaga gctcacgtgc
     1081 ctcaacgaag cttcggtgct gcacaacctc aaggagcgat actactcagg gcttatctac
     1141 acctattcag gcctgttctg tgtggtcatc aacccttata agaacctgcc catctactca
     1201 gaggagatcg tggagatgta caagggcaag aagaggcacg agatgccacc ccacatctac
     1261 gccatcacag atactgccta ccggagcatg atgcaggacc gggaagatca gtccatcctg
     1321 tgcacggggg agtctggagc agggaagaca gagaacacca agaaagtcat ccagtacctg
     1381 gcacatgtgg cctcctcaca caagagcaag aaggaccagg gggagttgga gcggcagctg
     1441 ctacaggcca accctatcct agaggccttt ggaaacgcca agacggtgaa gaatgacaac
     1501 tcctctcgat tcggtaaatt cattcgtatc aactttgatg tcaatggcta cattgttggt
     1561 gccaacattg agacttatct tctggagaaa tctcgtgcta tccgccaagc caaagaggag
     1621 cggaccttcc acatcttcta ctacctgctg tctggggccg gagaacacct gaagactgat
     1681 ctcctgttgg agccatacaa caaataccgc ttcctgtcca acgggcacgt caccatccct
     1741 gggcagcagg acaaggacat gttccaggag acaatggagg ccatgagaat tatgggtatc
     1801 ccagaggatg agcagatggg cttgctgcgg gtcatctctg gggtccttca gcttggcaac
     1861 attgccttca agaaggagcg gaacactgac caggcgtcca tgccggacaa cacagctgct
     1921 caaaaggtgt cccacctcct ggggatcaat gtgaccgact tcaccagagg catcctcacc
     1981 ccacgcatca aggtgggcag agactatgtg cagaaggcgc agactaaaga gcaggctgac
     2041 tttgccattg aggccttggc caaggctacc tatgagcgga tgttccgctg gctggtgctt
     2101 cgcatcaaca aagctctgga caagaccaag aggcagggcg cctcatttat cgggatactg
     2161 gacatcgctg gctttgaaat ctttgatctg aactccttcg agcagctgtg catcaactac
     2221 accaacgaga agctgcagca gctgttcaac cacaccatgt tcatcctgga gcaggaggag
     2281 taccagcgag agggcatcga gtggaacttc atcgacttcg gcctggacct gcagccctgc
     2341 atcgacctca ttgagaagcc ggcgggtccc ccaggcatcc tggccctgct agatgaggag
     2401 tgctggtttc ctaaggccac tgacaagagc ttcgtggaga aggtggtgca ggagcagggc
     2461 acccacccca agttccagaa gcccaagcaa ctgaaggaca aggctgattt ctgcattatc
     2521 cactatgccg gcaaggtgga ctataaagct gacgagtggc tgatgaagaa catggacccc
     2581 ttgaacgaca acatcgccac gctgcttcac cagtcctcag acaagtttgt ctctgagctg
     2641 tggaaggatg tggatcggat cattggcttg gaccaagtgg ctggaatgtc cgagacagca
     2701 ctacctggtg ccttcaagac ccggaagggc atgttccgta ctgtcggaca gctgtacaag
     2761 gagcagctgg ccaagctcat ggccacgttg aggaatacca accccaactt cgtgcgctgc
     2821 atcattccca accatgagaa gaaggccggc aaactggacc cgcacttggt gctggaccag
     2881 ctgcgctgca atggcgtcct tgagggcatc cggatctgcc gccagggctt tcccaacagg
     2941 gtggtcttcc aggagttccg gcagaggtat gagatcctca cccccaactc catcccgaag
     3001 ggcttcatgg atggcaagca agcgtgtgtg ctcatgatca aagccttgga gcttgacagc
     3061 aacctgtacc gcatcggcca gagcaaagtg ttcttccggg caggagtgct cgcccacctg
     3121 gaggaagagc gggacctgaa gatcaccgat gtcatcattg gcttccaggc ctgctgcagg
     3181 ggctacctgg ccaggaaggc ctttgccaag aggcagcaac agctgaccgc catgaaggtc
     3241 ctacagagga actgtgctgc gtacctcagg ctgcgcaact ggcagtggtg gaggctcttc
     3301 accaaggtca agcccctgtt gaactcaata agacatgagg atgagctgtt agccaaggag
     3361 gcggaactga caaaggttcg agagaaacat ctggctgcag agaacaggct gacagagatg
     3421 gagacgatgc agtctcagct catggcagag aagctgcagc ttcaggagca gctgcaggcg
     3481 gagacagagc tgtgtgccga ggctgaggag ctccgggccc gtctgacagc gaagaagcag
     3541 gagctggagg agatatgcca tgacctggag gccagggtgg aggaggagga ggagcgctgc
     3601 cagtacctgc aggccgagaa gaagaagatg cagcagaaca tccaggaact tgaggagcag
     3661 ttggaggagg aggagagcgc ccggcagaag ctgcagcttg agaaggtgac caccgaggcc
     3721 aagctgaaga aactggagga ggaccagatc atcatggagg accagaactg caaactggcc
     3781 aaggagaaga aactgctgga agacagagta gctgagttca ctaccaacct catggaagag
     3841 gaggagaagt ccaagagcct ggccaagctc aagaacaagc acgaggcaat gatcaccgac
     3901 ctggaagagc gcctccgtag ggaggagaag cagaggcagg agttggagaa gacccgtcgc
     3961 aagctggagg gagactccac agacctcagt gaccagattg ctgagctcca ggcgcagata
     4021 gcagagctca agatgcagct ggccaagaag gaggaggagt tgcaggctgc cttggccaga
     4081 gtggaagaag aagctgctca gaagaatatg gccctgaaga agatccgaga actggaaact
     4141 cagatatctg agctccagga ggacctggag tcggagcgag cctccaggaa taaagccgag
     4201 aagcagaaac gggatctggg agaggagctg gaggcgctga agacagagct ggaggacacg
     4261 ctggactcca cggctgccca gcaggagctg aggtcgaagc gtgagcagga ggtgagcatc
     4321 ctgaagaaga ctctggagga cgaggccaag acccatgagg cccagatcca ggagatgagg
     4381 cagaagcact cacaggctgt ggaggagctg gcagatcagt tggagcagac gaagcgggta
     4441 aaagctaccc ttgagaaggc gaagcagacc ctggagaatg agcggggaga gctggccaat
     4501 gaggtgaagg ccctgctgca aggcaagggc gactcagagc acaagcgcaa gaaggtggag
     4561 gcgcagctgc aagaactgca ggtcaagttc agcgagggag agcgcgtgcg aaccgaactg
     4621 gccgacaagg tcaccaagct gcaggttgaa ctggacagtg tgaccggtct ccttagccag
     4681 tctgacagca agtccagcaa gcttacgaag gacttctctg cgctggagtc ccagcttcag
     4741 gacacacagg agttgctcca ggaggagaac cggcagaagc tgagcctgag caccaagctc
     4801 aagcagatgg aggatgagaa aaactccttc agggagcagc tggaggagga ggaggaggcc
     4861 aagcgcaact tggagaagca gatcgccacg ctccatgccc aggtgaccga catgaagaag
     4921 aagatggagg acggtgtagg gtgcctggag actgcagagg aggcgaagcg gaggcttcag
     4981 aaggacttgg aaggcctgag ccagcggctt gaggagaagg tggctgccta cgataagctg
     5041 gagaagacca agacacggct gcagcaggag ctggacgacc tgctggttga cctggaccac
     5101 cagcggcaga gcgtctccaa cctggaaaag aagcagaaga agttcgacca gctcctagcc
     5161 gaggagaaga ccatctcggc caagtatgca gaggagcgtg accgagctga ggctgaggcc
     5221 cgtgagaagg agacaaaggc gctatcactg gcccgggcgc ttgaggaggc catggagcag
     5281 aaggcagagc tggagcggct caacaagcag ttccgcacgg agatggagga cctcatgagc
     5341 tccaaggatg acgtgggcaa gagtgtccac gagctggaga agtccaagcg ggccttggag
     5401 cagcaggtgg aggagatgaa gacccagctg gaggagctgg aggatgagct gcaggccacg
     5461 gaggatgcca agctccgcct ggaggtgaac ctgcaggcca tgaaggccca gtttgagcgg
     5521 gatctgcagg gccgggatga acagagcgag gagaagaaga agcagctggt cagacaggtg
     5581 cgggagatgg aggcggagct ggaggatgag aggaagcagc gctccatggc catggccgca
     5641 cgcaagaaac tggagatgga tctgaaggac ctggaggcac acattgacac agccaataag
     5701 aaccgggaag aggccatcaa acagctgcgg aagcttcagg cccagatgaa ggactgcatg
     5761 cgggagctgg acgacacgcg cgcctcccgg gaggagatcc tggcgcaggc caaggagaat
     5821 gagaagaagc tgaagagcat ggaggccgag atgattcagc tgcaggagga actggcagct
     5881 gctgagcgtg ctaagcgtca ggcccaacag gaacgggacg agctggctga tgagatcgcc
     5941 aacagcagtg gcaaaggggc cctagcatta gaggagaagc ggcgactgga ggcccgcatt
     6001 gccctgctgg aggaggagct ggaggaggaa cagggcaaca cggagctgat caacgatcgg
     6061 ctgaagaagg ccaacctgca gatcgaccaa ataaacaccg acctgaacct ggaacgcagc
     6121 cacgcacaga agaatgagaa tgcgcgacag cagctggaac gccagaacaa ggagctcaag
     6181 gccaagctgc aggaaatgga gagtgctgtc aagtccaaat acaaggcctc catcgcggcc
     6241 ttggaggcca aaattgcaca gctggaggaa cagctggaca acgagaccaa ggagcgccag
     6301 gcagcctcca agcaggtgcg ccggacggag aagaagctga aggacgtgct gctgcaggtg
     6361 gaggacgagc ggaggaacgc ggaacagttc aaggaccagg ctgacaaggc gtccacccgc
     6421 ctgaagcagc ttaaacggca gctagaggag gctgaagagg aggcccagcg ggccaatgcc
     6481 tcacgccgga agctgcagcg tgagctggaa gatgccacag agaccgctga tgctatgaac
     6541 cgcgaggtca gctccctgaa gaacaaactg aggcgtgggg acctgccatt tgtcgtgact
     6601 cgccgaattg ttcggaaagg cactggcgac tgctcagacg aggaggtcga cggtaaagca
     6661 gatggggccg atgccaaggc agctgaatag gaattctgca gtcgacggta ccgcgggccc
     6721 gggatccacc ggatctagat aactgatcat aatcagccat acca
//
