LOCUS       sequence-87270-e        2208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..761
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(90..111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     698..719
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcatgctagt gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      121 acaagttcag cgtccgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
      241 gctacggcgt ggcctgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      301 agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctctttcaag gacgacggta
      361 cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      481 tcaacagcca caacgtctat atcacggccg acaagcagaa gaacggcatc aaggctaact
      541 tcaagatccg ccacaacgtt gaggacggca gcgtgcagct cgccgaccac taccagcaga
      601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agccatcagt
      661 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      721 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatctggca
      781 gcggtggagg cagcgcatcc ggcggaagcg gaagcgtgcg tgagtgcatc tccatccacg
      841 ttggccaggc tggtgtccag attggcaatg cctgctggga gctctactgc ctggaacacg
      901 gcatccagcc cgatggccag atgccaagtg acaagaccat tgggggagga gatgactcct
      961 tcaacacctt cttcagtgag acgggcgctg gcaagcacgt gccccgggct gtgtttgtag
     1021 acttggaacc cacagtcatt gatgaagttc gcactggcac ctaccgccag ctcttccacc
     1081 ctgagcagct catcacaggc aaggaagatg ctgccaataa ctatgcccga gggcactaca
     1141 ccattggcaa ggagatcatt gaccttgtgt tggaccgaat tcgcaagctg gctgaccagt
     1201 gcaccggtct tcagggcttc ttggttttcc acagctttgg tgggggaact ggttctgggt
     1261 tcacctccct gctcatggaa cgtctctcag ttgattatgg caagaagtcc aagctggagt
     1321 tctccattta cccagcaccc caggtttcca cagctgtagt tgagccctac aactccatcc
     1381 tcaccaccca caccaccctg gagcactctg attgtgcctt catggtagac aatgaggcca
     1441 tctatgacat ctgtcgtaga aacctcgata tcgagcgccc aacctacact aaccttaacc
     1501 gccttattag ccagattgtg tcctccatca ctgcttccct gagatttgat ggagccctga
     1561 atgttgacct gacagaattc cagaccaacc tggtgcccta cccccgcatc cacttccctc
     1621 tggccacata tgcccctgtc atctctgctg agaaagccta ccatgaacag ctttctgtag
     1681 cagagatcac caatgcttgc tttgagccag ccaaccagat ggtgaaatgt gaccctcgcc
     1741 atggtaaata catggcttgc tgcctgttgt accgtggtga cgtggttccc aaagatgtca
     1801 atgctgccat tgccaccatc aaaaccaagc gcagcatcca gtttgtggat tggtgcccca
     1861 ctggcttcaa ggttggcatc aactaccagc ctcccactgt ggtgcctggt ggagacctgg
     1921 ccaaggtaca gagagctgtg tgcatgctga gcaacaccac agccattgct gaggcctggg
     1981 ctcgcctgga ccacaagttt gacctgatgt atgccaagcg tgcctttgtt cactggtacg
     2041 tgggtgaggg gatggaggaa ggcgagtttt cagaggcccg tgaagatatg gctgcccttg
     2101 agaaggatta tgaggaggtt ggtgtggatt ctgttgaagg agagggtgag gaagaaggag
     2161 aggaatacta aggatccacc ggatctagat aactgatcat aatcagcc
//
