LOCUS       sequence-87271-e        6015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..739
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(68..89)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     676..697
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             2440..2448
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
       61 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tccgcggcga
      121 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      181 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcgtgg cctgcttcag
      241 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      301 cgtccaggag cgcaccatct ctttcaagga cgacggtacc tacaagaccc gcgccgaggt
      361 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      421 ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat
      481 cacggccgac aagcagaaga acggcatcaa ggctaacttc aagatccgcc acaacgttga
      541 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      601 cgtgctgctg cccgacaacc actacctgag ccatcagtcc gccctgagca aagaccccaa
      661 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      721 catggacgag ctgtacaagt ccggaagtgc tgggagcgca agcggaagcg gaggctccgg
      781 tgaggaaaca gctatatggg aacaacatac agtgacgctt cacagggctc ctggatttgg
      841 atttggaatt gcaatatctg gtggacgaga taatcctcat tttcagagtg gggaaacgtc
      901 aatagtgatt tcagatgtgc tgaaaggagg accagctgaa ggacagctac aggaaaatga
      961 ccgagttgca atggttaacg gagtttcaat ggataatgtt gaacatgctt ttgctgttca
     1021 gcaactaagg aaaagtggga aaaatgcaaa aattacaatt agaaggaaga agaaagttca
     1081 aataccagta agtcgtcctg atcctgaacc agtatctgat aatgaagaag atagttatga
     1141 tgaggaaata catgatccaa gaagtggccg gagtggtgtg gttaacagaa ggagtgagaa
     1201 gatttggccg agggatagaa gtgcaagtag agagaggagc ttgtccccgc ggtcagacag
     1261 gcggtcagtg gcttccagcc agcctgctaa acctactaaa gtcacactgg tgaaatcccg
     1321 gaaaaatgaa gaatatggtc ttcgattggc aagccatata tttgttaagg aaatttcaca
     1381 agatagtttg gcagcaagag atggcaatat tcaagaaggt gatgttgtat tgaagataaa
     1441 tggtactgtg acagaaaata tgtcattgac agatgcaaag acattgatag aaaggtctaa
     1501 aggcaaatta aaaatggtag ttcaaagaga tgaacgggct acgctattga atgtccctga
     1561 tctttctgac agcatccact ctgctaatgc ctctgagaga gacgacattt cagaaattca
     1621 gtcactggca tcagatcatt ctggtcgatc acacgatagg cctccccgcc gcagccggtc
     1681 acgatctcct gaccagcggt cagagccttc tgatcattcc aggcactcgc cgcagcagcc
     1741 aagcaatggc agtctgcgga gtagagatga agagagaatt tctaaacctg gggctgtctc
     1801 aactcctgta aagcatgctg atgatcacac acctaaaaca gtggaagaag ttacagttga
     1861 aagaaatgag aaacaaacac cttctcttcc agaaccaaag cctgtgtatg cccaagttgg
     1921 caaccagatg tggatttacc tgtcagtcca tctgatggtg tcctacctaa ttcaactcat
     1981 gaagatggga tttcttcggc ccagcatgaa attggtaaaa ttcagaaaag gagatagtgt
     2041 gggtttgcgg ctggctggtg gaaatgatgt tggaatattt gtagctggcg ttctagaaga
     2101 tagccctgca gccaaggaag gcttagagga aggtgatcaa attctcaggg taaacaacgt
     2161 agattttaca aatatcataa gagaagaagc cgtccttttc ctgcttgacc tccctaaagg
     2221 agaagaagtg accatattgg ctcagaagaa gaaggatgtt tatcgtcgca ttgtagaatc
     2281 agatgtagga gattctttct atattagaac ccattttgaa tatgaaaagg aatctcccta
     2341 tggacttagt tttaacaaag gagaggtgtt ccgtgctgtg gataccttgt acaatggaaa
     2401 actgggctct tggcttgcta ttcgaattgg taaaaatcat aaggaggtag aacgaggcat
     2461 catccctaat aagaacagag ctgagcagct cgccagtgta cagtatacac ttccaaaaac
     2521 agcaggcgga gaccgtgctg acttctggag attcagaggt cttcgcagct ccaagagaaa
     2581 tcttcgaaaa agcagagagg atttgtccgc tcagcctgtt caaacaaagt ttccagctta
     2641 tgaaagagtg gttcttcgag aagctggatt tctgaggcct gtaaccattt ttggaccaat
     2701 agctgatgtt gccagagaaa agctggcaag agaagaacca gatatttatc aaattgcaaa
     2761 gagtgaacca cgagacgctg gaactgacca acgtagctct ggctatattc gcctgcatac
     2821 aataaagcaa atcatagatc aagacaaaca tgctttatta gatgtaacac caaatgcagt
     2881 tgatcgtctt aactatgccc agtggtatcc aattgttgta tttcttaacc ctgattctaa
     2941 gcaaggagta aaaacaatga gaatgaggtt atgtccagaa tctcggaaaa gtgccaggaa
     3001 gttatacgag cgatctcata aacttgctaa aaataatcac catcttttta caactacaat
     3061 taacttaaat tcaatgaatg atggttggta tggtgcgctg aaagaagcag ttcaacaaca
     3121 gcaaaaccag ctggtatggg tttccgaggg aaaggcggat ggtgctacaa gtgatgacct
     3181 tgatttgcat gatgatcgtc tgtcctacct gtcagctcca ggtagtgaat actcaatgta
     3241 tagcacggac agtagacaca cttctgacta tgaagacaca gacacagaag gcggggccta
     3301 cactgatcaa gaactagatg aaactcttaa tgatgaggtt gggactccac cggagtctgc
     3361 cattacacgg tcctctgagc ctgtaagaga ggactcctct ggaatgcatc atgaaaacca
     3421 aacatatcct ccttactcac cacaagcgca gccacaacca attcatagaa tagactcccc
     3481 tggatttaag ccagcctctc aacagaaagc agaagcttca tctccagtcc cttacctttc
     3541 gcctgaaaca aacccagcat catcaacctc tgctgttaat cataatgtaa atttaactaa
     3601 tgtcagactg gaggagccca ccccagctcc ttccacctct tactcaccac aagctgattc
     3661 tttaagaaca ccaagtactg aggcagctca cataatgcta agagatcaag aaccatcatt
     3721 gtcgtcgcat gtagatccaa caaaggtgta tagaaaggac ccatatcccg aggaaatgat
     3781 gaggcagaac catgttttga aacagccagc cgttagtcac ccagggcaca ggccagacaa
     3841 agagcctaat ctgacctatg aaccccaact cccatacgta gagaaacaag ccagcagaga
     3901 cctggagcag cccacataca gatacgagtc ctcaagctat acggaccagt tttctcgaaa
     3961 ctatgaacat cgtctgcgat acgaagatcg cgtccccatg tatgaagaac agtggtcata
     4021 ttatgatgac aaacagccct acccatctcg gccacctttt gataatcagc actctcaaga
     4081 ccttgactcc agacagcatc ccgaagagtc ctcagaacga gggtactttc cacgttttga
     4141 agagccagcc cctctgtctt acgacagcag accacgttac gaacaggcac ctagagcatc
     4201 cgccctgcgg cacgaagagc agccagctcc tgggtatgac acacatggta gactcagacc
     4261 ggaagcccag ccccaccctt cagcagggcc caagcctgca gagtccaagc agtattttga
     4321 gcaatattca cgcagttacg agcaagtacc accccaagga tttacctcta gagcaggtca
     4381 ttttgagcct ctccatggtg ctgcagctgt ccctccgctg ataccttcat ctcagcataa
     4441 gccagaagct ctgccttcaa acaccaaacc actgcctcca cccccaactc aaaccgaaga
     4501 agaggaagat ccagcaatga agccacagtc tgtactcacc agagttaaga tgtttgaaaa
     4561 caaaaggtct gcatccttag agaccaagaa ggatgtaaat gacactggca gttttaagcc
     4621 tccagaagta gcatctaaac cttcaggtgc tcccatcatt ggtcccaaac ccacttctca
     4681 gaatcaattc agtgaacatg acaaaactct gtacaggatt ccagaacctc aaaaacctca
     4741 actgaagcca cctgaagata ttgttcggtc caatcattat gaccctgaag aagatgaaga
     4801 atattatcga aaacagctgt catactttga ccgaagaagt tttgagaata agcctcctgc
     4861 acacattgct gccagccatc tctccgagcc tgcaaagcca gctcattctc agaatcaatc
     4921 aaatttttct agttattctt caaagggaaa gcctcctgaa gctgatggtg tggatagatc
     4981 atttggcgag aaacgctatg aacccatcca ggccactccc cctcctcctc cattgccctc
     5041 gcagtatgcc cagccatctc agcctgtcac cagcgcgtct ctccacatac attctaaggg
     5101 agcacatggt gaaggtaatt cagtgtcatt ggattttcag aattccttag tgtccaaacc
     5161 agacccacct ccatctcaga ataagccagc aactttcaga ccaccaaacc gagaagatac
     5221 tgctcaggca gctttctatc cccagaaaag ttttccagat aaagccccag ttaatggaac
     5281 tgaacagact cagaaaacag tcactccagc atacaatcga ttcacaccaa aaccatatac
     5341 aagttctgcc cgaccatttg aacgcaagtt tgaaagtcct aaattcaatc acaatcttct
     5401 gccaagtgaa actgcacata aacctgactt gtcttcaaaa actcccactt ctccaaaaac
     5461 tcttgtgaaa tcgcacagtt tggcacagcc tcctgagttt gacagtggag ttgaaacttt
     5521 ctctatccat gcagagaagc ctaaatatca aataaataat atcagcacag tgcctaaagc
     5581 tattcctgtg agtccttcag ctgtggaaga ggatgaagat gaagatggtc atactgtggt
     5641 ggccacagcc cgaggcatat ttaacagcaa tgggggcgtg ctgagttcca tagaaactgg
     5701 tgttagtata attatccctc aaggagccat tcccgaagga gttgagcagg aaatctattt
     5761 caaggtctgc cgggacaaca gcatccttcc acctttagat aaagagaaag gtgaaacact
     5821 gctgagtcct ttggtgatgt gtggtcccca tggcctcaag ttcctgaagc ctgtggagct
     5881 gcgcttacca cactgtgatc ctaaaacctg gcaaaacaag tgtcttcccg gagatccaaa
     5941 ttatctcgtt ggagcaaact gtgtttctgt ccttattgac cacttttaag gatccaccgg
     6001 atctagataa ctgat
//
