LOCUS       sequence-87273-e        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Clover2-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..743
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNSIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     680..701
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             774..1151
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtccgcg
      121 gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg
      181 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc gtggcctgct
      241 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      301 gctacgtcca ggagcgcacc atctctttca aggacgacgg tacctacaag acccgcgccg
      361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      421 aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc cactacgtct
      481 atatcacggc cgacaagcag aagaacagca tcaaggctaa cttcaagatc cgccacaacg
      541 ttgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      601 gccccgtgct gctgcccgac aaccactacc tgagccatca gtccgccctg agcaaagacc
      661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      721 tcggcatgga cgagctgtac aagtccggac tcagatctgg aggctccgga ggcccagagc
      781 cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga
      841 agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca
      901 aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg ggcatcatga
      961 attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt
     1021 acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc
     1081 ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca
     1141 gcgctaagta aggatccacc ggatctagat aactgatcat aatcagccat acc
//
