LOCUS       sequence-87275-e        1977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1977)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
      241 atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctggcag cggtggaggc
      781 agcgcatccg gcggaagcgg aagcgatgat gatatcgccg cgctcgtcgt cgacaacggc
      841 tccggcatgt gcaaggccgg cttcgcgggc gacgatgccc cccgggccgt cttcccctcc
      901 atcgtggggc gccccaggca ccagggcgtg atggtgggca tgggtcagaa ggattcctat
      961 gtgggcgacg aggcccagag caagagaggc atcctcaccc tgaagtaccc catcgagcac
     1021 ggcatcgtca ccaactggga cgacatggag aaaatctggc accacacctt ctacaatgag
     1081 ctgcgtgtgg ctcccgagga gcaccccgtg ctgctgaccg aggcccccct gaaccccaag
     1141 gccaaccgcg agaagatgac ccagatcatg tttgagacct tcaacacccc agccatgtac
     1201 gttgctatcc aggctgtgct atccctgtac gcctctggcc gtaccactgg catcgtgatg
     1261 gactccggtg acggggtcac ccacactgtg cccatctacg aggggtatgc cctcccccat
     1321 gccatcctgc gtctggacct ggctggccgg gacctgactg actacctcat gaagatcctc
     1381 accgagcgcg gctacagctt caccaccacg gccgagcggg aaatcgtgcg tgacattaag
     1441 gagaagctgt gctacgtcgc cctggacttc gagcaagaga tggccacggc tgcttccagc
     1501 tcctccctgg agaagagcta cgagctgcct gacggccagg tcatcaccat tggcaatgag
     1561 cggttccgct gccctgaggc actcttccag ccttccttcc tgggcatgga gtcctgtggc
     1621 atccacgaaa ctaccttcaa ctccatcatg aagtgtgacg tggacatccg caaagacctg
     1681 tacgccaaca cagtgctgtc tggcggcacc accatgtacc ctggcattgc cgacaggatg
     1741 cagaaggaga tcactgccct ggcacccagc acaatgaaga tcaagatcat tgctcctcct
     1801 gagcgcaagt actccgtgtg gatcggcggc tccatcctgg cctcgctgtc caccttccag
     1861 cagatgtgga tcagcaagca ggagtatgac gagtccggcc cctccatcgt ccaccgcaaa
     1921 tgcttctagg gatccaccgg atctagataa ctgatcataa tcagccatac cacattt
//
