LOCUS       sequence-87276-e        3550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 516, 
            Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3550)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(29..31,32..34,35..745)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     682..703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2564..2575
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg
      241 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      481 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaagc tgagcaaaga
      661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      721 tctcggcatg gacgagctgt acaagtccgg actcagatct ggcagcggtg gaggcttgga
      781 ggagccggag gccgcgacgc ggacagccgc ggccgtggat tgcaaagaca gacccgggtt
      841 cccagtgaag aggttaatac aagcccgctt gcccttcaaa cgcctgaacc ttgttcccaa
      901 ggagaaagtt gaggaagaca catcaccaaa agcagctgtg gaaagcaaag tcccggatct
      961 acaattgtct ttgggcactt ttgaaagcca gtgtcacaca ggctctcacg taggcttgag
     1021 tacaaagttg gtcggtggcc agggccccat tgatagcttc ttgagggcaa caatcaagcc
     1081 agtaccgagt gtggtcatta tcgacctgac ggagaactgc agtgacattc cagacagccc
     1141 tgagggccac agtgaactaa gtcctgacac tgctggtgtg gtcaccacag tggaaggagc
     1201 tgccaagcag caggagcact ctgcagcaga gctgtgtctc ctagagaccc cttcagacat
     1261 cacttgtcac atggaagagg agcctgggag tccaggagac ccaaagagga cgggtgactg
     1321 tcaggctggt tcactacaga gctgtccgga gctgacccca ggttctagga catgccccac
     1381 caaggagtta tcaagctgga gcaaggcagg ggatctccta tttatagaga aagtgcctgt
     1441 ggtggtgcta gaggacatcc tggccactaa gccctccatt gcctctcttc ccatgatgtc
     1501 cctggatagg agtgtcactt cagagagtga aatactggag tcctgccccg aggatgactc
     1561 catactgagc cactcctcca ccaactcctc ttctccaacc agctctcctg agggaccatc
     1621 cacaccccca gaacatcggg gtgggagaag ctccccctcc accccagcct gcagagttgc
     1681 caagaacttt gtcaaaggct ccacagagaa gggcaggagc aaactgcaca gagatagaga
     1741 acagcagagg gaagagaagg aaaagctgag ggaagagata cgacgagcca aggaggaggc
     1801 acggaagaag aaggaggagg agaaggagct gaaggaaaag gagcggaggg aaaagcggga
     1861 gaaagatgag aaggagaagg cggagaagca gcggctaaag gaggaaaaac gcaaggagcg
     1921 gcaggaagct ctggaggcta aactggagga gaagaggaag aaggaggaag agaagcggtt
     1981 acgggaggaa gagaagcggt tacgggaaga agagaagcgc attaaggcag aaaaggcaga
     2041 gatcactcga ttcttccaga aaccgaagac tccgcaggct cccaagaccc tggccggttc
     2101 ctgtggtaag tttgccccct ttgaaatcaa agaacacatg gtgctggctc cacggtgccg
     2161 agctgccttg gatcaagacc tgtgcgatca gctggaccag ctcctgcagc agcagagtgt
     2221 ggcaagcacc ttcctcagtg acctaaaaag ccggctgccc ttgagatcgg ggcccacccg
     2281 ggtctgtggc catgacacag acatcatgaa cagggatgtg gtcattgtgg agagcagcaa
     2341 ggtggacggt gtgtctgaaa ggaagaagtt tggccgcatg aagcttctgc agttctcaga
     2401 gaaccaccgg ccagcatact gggggacgtg gaacaagaag acagccatca tccgcccccg
     2461 gaacccctgg gcccaggaca aagaccttct tgactatgag gtagacagtg acgatgagtg
     2521 ggaagaggag gaacctgggg agtctttgtc ccacagtgag ggggatgagg atgacgatgt
     2581 gggggaagat gaggatgagg acgatggctt ctttgtgccc catgggtact tatctgaaga
     2641 tgaaggcgtg actgaggagt gtgcagaccc tgagaaccac aaagtacacc agaagctgaa
     2701 ggccaaggag tgggatgagc tcctagccaa gggcaagcgc tttcgtgtgc tgcagcctgt
     2761 gcacgtggga tgtgtatggg catcagaggc agccaactgc acgagttccg acttgaagct
     2821 gctgcagcag ttcactgcat gcctgctgga tgtggcatcc cctgatgagc cagaacccgg
     2881 ggcctccagg agggaaaaga gggaccagca tatcctggcc cagttgcttc cgctgctaca
     2941 tggcaatgtg aatgggagca aggtgatcat ccatgaattc caggagcaat gccgcagggg
     3001 cctgctgacc cttccaagtc ccaccccaca cctgcagatg cccaacttgg aggatgctgt
     3061 tgctgtgcca tccaaggcca ggctgaagcg ccttatctct gagaactctg cctatgagaa
     3121 gcggcccaac ttccgtatgt gctggtatgt gcacccagag gtgctcaaga gctttggcca
     3181 ggagtgcctg ccagtgccct gccagtggac ctacatcacc accatgccct cagcacccag
     3241 ggaagacagt ggcagtgctt ctactgaggg gcctggccag agcacaccta tgctactcaa
     3301 gcggaagcca gcggccacca tgtgcatcac gcagtttatg aagaagcggc gctatgatgg
     3361 acaggttggg tctggggaca tggatggctt ccaggcagac acagaagagg atgaagagga
     3421 tgacacagac tgtatgatca tagatgtgcc agatgttggg agtgacgtgt cagaagctcc
     3481 catacctgct cccacacttt gcaagtgagg atccaccgga tctagataac tgatcataat
     3541 cagccatacc
//
