LOCUS       sequence-87278-e        1961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1961)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(19..21,22..24,25..735)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(64..85)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(325..344)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     672..693
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc
      241 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc
      481 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
      541 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagtccgg actcagatct ggcagcggtg gaggcagcgc atccggcgga
      781 agcggaagcg atgatgatat cgccgcgctc gtcgtcgaca acggctccgg catgtgcaag
      841 gccggcttcg cgggcgacga tgccccccgg gccgtcttcc cctccatcgt ggggcgcccc
      901 aggcaccagg gcgtgatggt gggcatgggt cagaaggatt cctatgtggg cgacgaggcc
      961 cagagcaaga gaggcatcct caccctgaag taccccatcg agcacggcat cgtcaccaac
     1021 tgggacgaca tggagaaaat ctggcaccac accttctaca atgagctgcg tgtggctccc
     1081 gaggagcacc ccgtgctgct gaccgaggcc cccctgaacc ccaaggccaa ccgcgagaag
     1141 atgacccaga tcatgtttga gaccttcaac accccagcca tgtacgttgc tatccaggct
     1201 gtgctatccc tgtacgcctc tggccgtacc actggcatcg tgatggactc cggtgacggg
     1261 gtcacccaca ctgtgcccat ctacgagggg tatgccctcc cccatgccat cctgcgtctg
     1321 gacctggctg gccgggacct gactgactac ctcatgaaga tcctcaccga gcgcggctac
     1381 agcttcacca ccacggccga gcgggaaatc gtgcgtgaca ttaaggagaa gctgtgctac
     1441 gtcgccctgg acttcgagca agagatggcc acggctgctt ccagctcctc cctggagaag
     1501 agctacgagc tgcctgacgg ccaggtcatc accattggca atgagcggtt ccgctgccct
     1561 gaggcactct tccagccttc cttcctgggc atggagtcct gtggcatcca cgaaactacc
     1621 ttcaactcca tcatgaagtg tgacgtggac atccgcaaag acctgtacgc caacacagtg
     1681 ctgtctggcg gcaccaccat gtaccctggc attgccgaca ggatgcagaa ggagatcact
     1741 gccctggcac ccagcacaat gaagatcaag atcattgctc ctcctgagcg caagtactcc
     1801 gtgtggatcg gcggctccat cctggcctcg ctgtccacct tccagcagat gtggatcagc
     1861 aagcaggagt atgacgagtc cggcccctcc atcgtccacc gcaaatgctt ctagggatcc
     1921 accggatcta gataactgat cataatcagc cataccacat t
//
