LOCUS       sequence-87279-e        1784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1784)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-ATG3-C-18
FEATURES             Location/Qualifiers
     source          1..1784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(338..357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca
      241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      481 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      541 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctagtagcg gaggtagcgc
      781 atccggcgga agcggaggaa gccagaatgt gatcaacacg gtgaagggaa aggctctgga
      841 agtggccgag tacctgaccc cggtcctcaa ggaatcaaaa tttaaggaaa cgggtgtaat
      901 cactccagaa gagtttgtgg cagctggaga tcacttagtc caccactgtc caacatggca
      961 atgggctaca ggggaagaat tgaaagtgaa ggcatatctt ccgacagaca aacaattttt
     1021 ggtaaccaaa aatgttccat gctacaagcg gtgtaaacag atggagtatt cggatgaatt
     1081 ggaagctatc attgaagaag atgatggtga tgggggatgg gtagatacat atcacaacac
     1141 aggtattaca ggaattactg aagcagttaa ggagattaca ctggaaagca aggacagtat
     1201 aaaactccaa gattgctcag cactgtgtga tgaagaagac gaggaagatg aaggggaagc
     1261 tgcagacatg gaagaatatg aagagagtgg attgttggaa acagatgagg ctaccctaga
     1321 cacaaggaaa atagtggaag cctgcaaagc taaggctgac gctggaggtg aagatgctat
     1381 tttacaaacg agaacatacg atctgtacat cacttacgac aaatattacc agacaccacg
     1441 gctatggttg tttggctatg atgagcaacg gcagccttta acagttgagc acatgtatga
     1501 agacatcagt caagatcatg tgaagaaaac agtgaccatt gaaaaccatc ctcatctccc
     1561 accacctcct atgtgttcag ttcacccatg caggcatgct gaagtgatga agaaaattat
     1621 tgagacagtt gcagaaggcg ggggagagct tggtgttcat atgtatcttt taattttttt
     1681 gaaatttgtt caagctgtca ttccaacaat agaatatgac tacacaagac acttcacaat
     1741 gtagggatcc accggatcta gataactgat cataatcagc cata
//
