LOCUS       sequence-87283-e        2048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2048)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..2048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      541 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctggtgg ctccgcagga
      781 tcccggcaag aagaagcaaa ggctgctgta ggtgtgactg gaaatgacat cactaccccg
      841 ccaaacaagg agccaccacc aagcccagaa aagaaagcaa agcctttggc caccactcaa
      901 cctgcaaaga cttcaacatc gaaagccaaa acacagccca cttctctccc taagcaacca
      961 gctcccacca cctctggtgg gttgaataaa aaacccatga gcctcgcctc aggctcagtg
     1021 ccagctgccc cacacaaacg ccctgctgct gccactgcta ctgccaggcc ttccacccta
     1081 cctgccagag acgtgaagcc aaagccaatt acagaagcta aggttgccga aaagcggacc
     1141 tctccatcca agccttcatc tgccccagcc ctcaaacctg gacctaaaac caccccaacc
     1201 gtttcaaaag ccacatctcc ctcaactctt gtttccactg gaccaagtag tagaagtcca
     1261 gctacaactc tgcctaagag gccaaccagc atcaagactg aggggaaacc tgctgatgtc
     1321 aaaaggatga ctgctaagtc tgcctcagct gacttgagtc gctcaaagac cacctctgcc
     1381 agttctgtga agagaaacac cactcccact ggggcagcac ccccagcagg gatgacttcc
     1441 actcgagtca agcccatgtc tgcacctagc cgctcttctg gggctctttc tgtggacaag
     1501 aagcccactt ccactaagcc tagctcctct gctcccaggg tgagccgcct ggccacaact
     1561 gtttctgccc ctgacctgaa gagtgttcgc tccaaggtcg gctctacaga aaacatcaaa
     1621 caccagcctg gaggaggccg ggccaaggta gagaaaaaaa cagaggcagc taccacagct
     1681 gggaagcctg aacctaatgc agtcactaaa gcagccggct ccattgcgag tgcacagaaa
     1741 ccgcctgctg ggaaagtcca gatagtatcc aaaaaagtga gctacagtca tattcaatcc
     1801 aagtgtgttt ccaaggacaa tattaagcat gtccctggat gtggcaatgt tcagattcag
     1861 aacaagaaag tggacatatc caaggtctcc tccaagtgtg ggtccaaagc taatatcaag
     1921 cacaagcctg gtggaggaga tgtcaagatt gaaagtcaga agttgaactt caaggagaag
     1981 gcccaagcca aagtgggata gggatccacc ggatctagat aactgatcat aatcagccat
     2041 accacatt
//
