LOCUS       sequence-87284-e        6763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6763)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(38..40,41..43,44..754)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgaccgctc gagccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
      241 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      481 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
      541 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
      601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct
      661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      721 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctg gcagcggtgg
      781 aggcagcgca tccggcggaa gcggaagcgc tcagcaggct gcagacaagt acctctatgt
      841 ggataaaaac ttcatcaata acccgctggc ccaagctgac tgggctgcca agaagttggt
      901 atgggtgcct tccagcaaga atggctttga accagccagc ctcaaggagg aggtgggaga
      961 agaggccatt gtagagctgg tagagaatgg gaagaaggtg aaggtgaaca aggacgacat
     1021 ccagaagatg aacccaccca agttctccaa ggtggaggac atggcagagc tcacgtgcct
     1081 caacgaagct tcggtgctgc acaacctcaa ggagcgatac tactcagggc ttatctacac
     1141 ctattcaggc ctgttctgtg tggtcatcaa cccttataag aacctgccca tctactcaga
     1201 ggagatcgtg gagatgtaca agggcaagaa gaggcacgag atgccacccc acatctacgc
     1261 catcacagat actgcctacc ggagcatgat gcaggaccgg gaagatcagt ccatcctgtg
     1321 cacgggggag tctggagcag ggaagacaga gaacaccaag aaagtcatcc agtacctggc
     1381 acatgtggcc tcctcacaca agagcaagaa ggaccagggg gagttggagc ggcagctgct
     1441 acaggccaac cctatcctag aggcctttgg aaacgccaag acggtgaaga atgacaactc
     1501 ctctcgattc ggtaaattca ttcgtatcaa ctttgatgtc aatggctaca ttgttggtgc
     1561 caacattgag acttatcttc tggagaaatc tcgtgctatc cgccaagcca aagaggagcg
     1621 gaccttccac atcttctact acctgctgtc tggggccgga gaacacctga agactgatct
     1681 cctgttggag ccatacaaca aataccgctt cctgtccaac gggcacgtca ccatccctgg
     1741 gcagcaggac aaggacatgt tccaggagac aatggaggcc atgagaatta tgggtatccc
     1801 agaggatgag cagatgggct tgctgcgggt catctctggg gtccttcagc ttggcaacat
     1861 tgccttcaag aaggagcgga acactgacca ggcgtccatg ccggacaaca cagctgctca
     1921 aaaggtgtcc cacctcctgg ggatcaatgt gaccgacttc accagaggca tcctcacccc
     1981 acgcatcaag gtgggcagag actatgtgca gaaggcgcag actaaagagc aggctgactt
     2041 tgccattgag gccttggcca aggctaccta tgagcggatg ttccgctggc tggtgcttcg
     2101 catcaacaaa gctctggaca agaccaagag gcagggcgcc tcatttatcg ggatactgga
     2161 catcgctggc tttgaaatct ttgatctgaa ctccttcgag cagctgtgca tcaactacac
     2221 caacgagaag ctgcagcagc tgttcaacca caccatgttc atcctggagc aggaggagta
     2281 ccagcgagag ggcatcgagt ggaacttcat cgacttcggc ctggacctgc agccctgcat
     2341 cgacctcatt gagaagccgg cgggtccccc aggcatcctg gccctgctag atgaggagtg
     2401 ctggtttcct aaggccactg acaagagctt cgtggagaag gtggtgcagg agcagggcac
     2461 ccaccccaag ttccagaagc ccaagcaact gaaggacaag gctgatttct gcattatcca
     2521 ctatgccggc aaggtggact ataaagctga cgagtggctg atgaagaaca tggacccctt
     2581 gaacgacaac atcgccacgc tgcttcacca gtcctcagac aagtttgtct ctgagctgtg
     2641 gaaggatgtg gatcggatca ttggcttgga ccaagtggct ggaatgtccg agacagcact
     2701 acctggtgcc ttcaagaccc ggaagggcat gttccgtact gtcggacagc tgtacaagga
     2761 gcagctggcc aagctcatgg ccacgttgag gaataccaac cccaacttcg tgcgctgcat
     2821 cattcccaac catgagaaga aggccggcaa actggacccg cacttggtgc tggaccagct
     2881 gcgctgcaat ggcgtccttg agggcatccg gatctgccgc cagggctttc ccaacagggt
     2941 ggtcttccag gagttccggc agaggtatga gatcctcacc cccaactcca tcccgaaggg
     3001 cttcatggat ggcaagcaag cgtgtgtgct catgatcaaa gccttggagc ttgacagcaa
     3061 cctgtaccgc atcggccaga gcaaagtgtt cttccgggca ggagtgctcg cccacctgga
     3121 ggaagagcgg gacctgaaga tcaccgatgt catcattggc ttccaggcct gctgcagggg
     3181 ctacctggcc aggaaggcct ttgccaagag gcagcaacag ctgaccgcca tgaaggtcct
     3241 acagaggaac tgtgctgcgt acctcaggct gcgcaactgg cagtggtgga ggctcttcac
     3301 caaggtcaag cccctgttga actcaataag acatgaggat gagctgttag ccaaggaggc
     3361 ggaactgaca aaggttcgag agaaacatct ggctgcagag aacaggctga cagagatgga
     3421 gacgatgcag tctcagctca tggcagagaa gctgcagctt caggagcagc tgcaggcgga
     3481 gacagagctg tgtgccgagg ctgaggagct ccgggcccgt ctgacagcga agaagcagga
     3541 gctggaggag atatgccatg acctggaggc cagggtggag gaggaggagg agcgctgcca
     3601 gtacctgcag gccgagaaga agaagatgca gcagaacatc caggaacttg aggagcagtt
     3661 ggaggaggag gagagcgccc ggcagaagct gcagcttgag aaggtgacca ccgaggccaa
     3721 gctgaagaaa ctggaggagg accagatcat catggaggac cagaactgca aactggccaa
     3781 ggagaagaaa ctgctggaag acagagtagc tgagttcact accaacctca tggaagagga
     3841 ggagaagtcc aagagcctgg ccaagctcaa gaacaagcac gaggcaatga tcaccgacct
     3901 ggaagagcgc ctccgtaggg aggagaagca gaggcaggag ttggagaaga cccgtcgcaa
     3961 gctggaggga gactccacag acctcagtga ccagattgct gagctccagg cgcagatagc
     4021 agagctcaag atgcagctgg ccaagaagga ggaggagttg caggctgcct tggccagagt
     4081 ggaagaagaa gctgctcaga agaatatggc cctgaagaag atccgagaac tggaaactca
     4141 gatatctgag ctccaggagg acctggagtc ggagcgagcc tccaggaata aagccgagaa
     4201 gcagaaacgg gatctgggag aggagctgga ggcgctgaag acagagctgg aggacacgct
     4261 ggactccacg gctgcccagc aggagctgag gtcgaagcgt gagcaggagg tgagcatcct
     4321 gaagaagact ctggaggacg aggccaagac ccatgaggcc cagatccagg agatgaggca
     4381 gaagcactca caggctgtgg aggagctggc agatcagttg gagcagacga agcgggtaaa
     4441 agctaccctt gagaaggcga agcagaccct ggagaatgag cggggagagc tggccaatga
     4501 ggtgaaggcc ctgctgcaag gcaagggcga ctcagagcac aagcgcaaga aggtggaggc
     4561 gcagctgcaa gaactgcagg tcaagttcag cgagggagag cgcgtgcgaa ccgaactggc
     4621 cgacaaggtc accaagctgc aggttgaact ggacagtgtg accggtctcc ttagccagtc
     4681 tgacagcaag tccagcaagc ttacgaagga cttctctgcg ctggagtccc agcttcagga
     4741 cacacaggag ttgctccagg aggagaaccg gcagaagctg agcctgagca ccaagctcaa
     4801 gcagatggag gatgagaaaa actccttcag ggagcagctg gaggaggagg aggaggccaa
     4861 gcgcaacttg gagaagcaga tcgccacgct ccatgcccag gtgaccgaca tgaagaagaa
     4921 gatggaggac ggtgtagggt gcctggagac tgcagaggag gcgaagcgga ggcttcagaa
     4981 ggacttggaa ggcctgagcc agcggcttga ggagaaggtg gctgcctacg ataagctgga
     5041 gaagaccaag acacggctgc agcaggagct ggacgacctg ctggttgacc tggaccacca
     5101 gcggcagagc gtctccaacc tggaaaagaa gcagaagaag ttcgaccagc tcctagccga
     5161 ggagaagacc atctcggcca agtatgcaga ggagcgtgac cgagctgagg ctgaggcccg
     5221 tgagaaggag acaaaggcgc tatcactggc ccgggcgctt gaggaggcca tggagcagaa
     5281 ggcagagctg gagcggctca acaagcagtt ccgcacggag atggaggacc tcatgagctc
     5341 caaggatgac gtgggcaaga gtgtccacga gctggagaag tccaagcggg ccttggagca
     5401 gcaggtggag gagatgaaga cccagctgga ggagctggag gatgagctgc aggccacgga
     5461 ggatgccaag ctccgcctgg aggtgaacct gcaggccatg aaggcccagt ttgagcggga
     5521 tctgcagggc cgggatgaac agagcgagga gaagaagaag cagctggtca gacaggtgcg
     5581 ggagatggag gcggagctgg aggatgagag gaagcagcgc tccatggcca tggccgcacg
     5641 caagaaactg gagatggatc tgaaggacct ggaggcacac attgacacag ccaataagaa
     5701 ccgggaagag gccatcaaac agctgcggaa gcttcaggcc cagatgaagg actgcatgcg
     5761 ggagctggac gacacgcgcg cctcccggga ggagatcctg gcgcaggcca aggagaatga
     5821 gaagaagctg aagagcatgg aggccgagat gattcagctg caggaggaac tggcagctgc
     5881 tgagcgtgct aagcgtcagg cccaacagga acgggacgag ctggctgatg agatcgccaa
     5941 cagcagtggc aaaggggccc tagcattaga ggagaagcgg cgactggagg cccgcattgc
     6001 cctgctggag gaggagctgg aggaggaaca gggcaacacg gagctgatca acgatcggct
     6061 gaagaaggcc aacctgcaga tcgaccaaat aaacaccgac ctgaacctgg aacgcagcca
     6121 cgcacagaag aatgagaatg cgcgacagca gctggaacgc cagaacaagg agctcaaggc
     6181 caagctgcag gaaatggaga gtgctgtcaa gtccaaatac aaggcctcca tcgcggcctt
     6241 ggaggccaaa attgcacagc tggaggaaca gctggacaac gagaccaagg agcgccaggc
     6301 agcctccaag caggtgcgcc ggacggagaa gaagctgaag gacgtgctgc tgcaggtgga
     6361 ggacgagcgg aggaacgcgg aacagttcaa ggaccaggct gacaaggcgt ccacccgcct
     6421 gaagcagctt aaacggcagc tagaggaggc tgaagaggag gcccagcggg ccaatgcctc
     6481 acgccggaag ctgcagcgtg agctggaaga tgccacagag accgctgatg ctatgaaccg
     6541 cgaggtcagc tccctgaaga acaaactgag gcgtggggac ctgccatttg tcgtgactcg
     6601 ccgaattgtt cggaaaggca ctggcgactg ctcagacgag gaggtcgacg gtaaagcaga
     6661 tggggccgat gccaaggcag ctgaatagga attctgcagt cgacggtacc gcgggcccgg
     6721 gatccaccgg atctagataa ctgatcataa tcagccatac cac
//
