LOCUS       sequence-87285-e        2334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2334)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      541 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggaagt gctgggagcg caagcggaag
      781 cggaggctcc ggttttaact acgaacgtcc aaaacacttc atccagtccc aaaacccatg
      841 tggctccaga ttgcagcctc ctggaccaga aacctccagc ttctctagcc agaccaaaca
      901 gtcttccatt atcatccagc cccgccagtg tacagagcaa agattttctg cctcctcaac
      961 actgagctct cacatcacca tgtcctcctc tgctttccct gcttctcccc agcagcatgc
     1021 tggctccaac ccaggccaaa gggttacaac cacctataac cagtccccag ccagcttcct
     1081 cagctccata ttaccatcac agcctgatta caatagcagt aaaatccctt ccgctatgga
     1141 ttccaactat caacagtcct cagctggcca acctataaat gcaaagccat cccaaactgc
     1201 aaatgctaag cccataccaa gaactcctga tcatgaaata caaggatcaa aagaagcttt
     1261 gattcaagat ttggaaagaa agctgaaatg caaggacacc cttcttcata atggaaatca
     1321 acgtctaaca tatgaagaga agatggctcg cagattgcta ggaccacaga atgcagctgc
     1381 tgtgtttcaa gctcaggatg acagtggtgc acaagactcg cagcaacaca actcagaaca
     1441 tgcgcgactg caagttccta catcacaagt aagaagtaga tcaacctcaa ggggagatgt
     1501 gaatgatcag gatgcaatcc aggagaaatt ttacccacca cgtttcattc aagtgccaga
     1561 gaacatgtcg attgatgaag gaagattctg cagaatggac ttcaaagtga gtggactgcc
     1621 agctcctgat gtgtcatggt atctaaatgg aagaacagtt caatcagatg atttgcacaa
     1681 aatgatagtg tctgagaagg gtcttcattc actcatcttt gaagtagtca gagcttcaga
     1741 tgcaggggct tatgcatgtg ttgccaagaa tagagcagga gaagccacct tcactgtgca
     1801 gctggatgtc cttgcaaaag aacataaaag agcaccaatg tttatctaca aaccacagag
     1861 caaaaaagtt ttagagggag attcagtgaa actagaatgc cagatctcgg ctatacctcc
     1921 accaaagctt ttctggaaaa gaaataatga aatggtacaa ttcaacactg accgaataag
     1981 cttatatcaa gataacactg gaagagttac tttactgata aaagatgtaa acaagaaaga
     2041 tgctgggtgg tatactgtgt cagcagttaa tgaagctgga gtgactacat gtaacacaag
     2101 attagacgtt acggcacgtc caaaccaaac tcttccagct cctaagcagt tacgggttcg
     2161 accaacattc agcaaatatt tagcacttaa tgggaaaggt ttgaatgtaa aacaagcttt
     2221 taacccagaa ggagaatttc agcgtttggc agctcaatct ggactctatg aaagtgaaga
     2281 actttaagga tccaccggat ctagataact gatcataatc agccatacca catt
//
