LOCUS       sequence-87286-e        2854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2854)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..2854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(38..40,41..43,44..754)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
      241 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      481 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
      541 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
      601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct
      661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      721 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gagtgagcgc
      781 tctggcctcc cgcgctaccg ccatgcagtc ctccggctcc ttcaactacg cgcgccctaa
      841 gcagttcatc gcggcgcaga acctgggtcc cgcatcgggg ctgcccacgc caacctccag
      901 ccccagctcc tccagcttgc cgtcaccatt gtcccccacg cccaggccct tcggccgcgc
      961 gcccgggccg cccttcgtgg aacccgaggc catgtggggc ccgtcctcgc cctcgccgcc
     1021 accgccgcca cctccggtct tcagcccctc tgcagcctac ccggtgcccg acgtgttccc
     1081 actgccaccg ccgccgccac cgctacctag ctccacctca cactgcgcct cgcccgcccg
     1141 cttcggcccc agccagacgc ccgcagcctt cctcagcgcc ctgctgccct ctcagccacc
     1201 gcctgtcgct gtcaacgccc tggggctgcc caagggagtc acccccgcgg ggttcccaaa
     1261 gaagtccagt agaactgcta gaattgcctc tgatgaggag attcaaggca caaaggatgc
     1321 tgtcatccaa gacctggaac ggaagcttcg cttcaaggag gaccttctga acaatggcca
     1381 accgaggcta acctatgagg aaagaatggc tcgccgcctg cttggagccg acagcgcaaa
     1441 cgtcttcaac atccaggagc cagaggaaac ggcagccaat caggagtaca aagtctctag
     1501 ctgcgagcag aggctgatta gcgagattga atacaggctg gagcgctccc ctgtggatga
     1561 gtcgggagac gaggtgcagg atccagatgt gcctgtggag aacgcaacag ctcccttctt
     1621 tgagatgaag ctgaaacact acaagatctt tgaggggatg ccggtgactt tcacgtgtcg
     1681 agtggctggg aatccaaagc caaagatcta ttggtttaaa gatgggaagc agatttctcc
     1741 gaagagcgat cactacacca ttcagagaga ccttgatggg acctgctctc tccacaccac
     1801 ggcctctacc ctagacgacg atgggaacta caccatcatg gctgccaacc ctcagggtcg
     1861 cgtcagttgt acaggaaggc taatggtaca ggctgtcaac caaagaggcc gcagtccccg
     1921 ctctccctca ggccatcctc atgccagaag gcctcgctct cgatcacggg acagtggaga
     1981 tgaaaacgag cccattcagg agcgattctt cagacctcac ttcctgcagg ctcctggaga
     2041 cctgaccgtt caggaaggca agctctgcag gatggactgc aaggtcagtg ggttaccaac
     2101 cccagatctc agctggcaac tagacggaaa gcccatacgc cccgacagtg ctcacaagat
     2161 gctggtccgt gagaatgggg tccactccct cattatagag ccagtcacgt cccgggacgc
     2221 cggcatctac acatgtattg ccaccaacag agcaggacag aactcgttta acctggagct
     2281 tgtggttgct gctaaggaag cacacaaggc ccctgtgttt atggagaagc tacagaacac
     2341 gggggttgct gatggatacc cagtgcggct agaatgccgt gtctccggag tgccgccacc
     2401 tcagatattt tggaagaaag aaaatgaatc gctcactcat agcactgagc gagtaagcat
     2461 gcaccaggat aatcatggct acatctgcct gctcatccag ggagccacaa aggaagacgc
     2521 tgggtggtac actgtgtctg ccaagaacga agcaggcatt gtgtcctgca ctgcccggct
     2581 ggatgtctac acccagtggc accagcagcc acagaccacc aagccaaaaa aagtacggcc
     2641 ctcggccagt cgctacgcag cactttcgga ccagggacta gacatcaaag ccgctttcca
     2701 acctgaagcc agcccatctc acctgactct gaacagcggc ttggtagaaa gtgaagacct
     2761 gtgagaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
     2821 tcataatcag ccataccaca ttgtagagtt ttag
//
