LOCUS       sequence-87287-e        1952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1952)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Sequestosome1-C-18
FEATURES             Location/Qualifiers
     source          1..1952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      481 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
      541 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga gagagcggag
      781 gtagcgcatc cggcggaagc ggaggagcca tgtcctacgt gaaggatgac atcttccgaa
      841 tctacattaa agagaaaaaa gagtgccggc gggaccaccg cccaccgtgt gctcaggagg
      901 cgccccgcaa catggtgcac cccaatgtga tctgcgatgg ctgcaatggg cctgtggtag
      961 gaacccgcta caagtgcagc gtctgcccag actacgactt gtgtagcgtc tgcgagggaa
     1021 agggcttgca ccgggggcac accaagctcg cattccccag ccccttcggg cacctgtctg
     1081 agggcttctc gcacagccgc tggctccgga aggtgaaaca cggacacttc gggtggccag
     1141 gatgggaaat gggtccacca ggaaactgga gcccacgtcc tcctcgtgca ggggaggccc
     1201 gccctggccc cacggcagaa tcagcttctg gtccatcgga ggatccgagt gtgaatttcc
     1261 tgaagaacgt tggggagagt gtggcagctg cccttagccc tctgggcatt gaagttgata
     1321 tcgatgtgga gcacggaggg aaaagaagcc gcctgacccc cgtctctcca gagagttcca
     1381 gcacagagga gaagagcagc tcacagccaa gcagctgctg ctctgatccc agcaagccgg
     1441 gtgggaatgt tgagggcgcc acgcagtctc tggcggagca gatgagaaag atcgccttgg
     1501 agtccgaggg gcgccctgag gaacagatgg agtcggataa ctgttcagga ggagatgatg
     1561 actggaccca tctgtcttca aaagaagtgg acccgtctac aggtgaactc cagtccctac
     1621 agatgccaga atccgaaggg ccaagctctc tggacccctc ccaggaggga cccacagggc
     1681 tgaaggaagc tgccttgtac ccacatctcc cgccagaggc tgacccgcgg ctgattgagt
     1741 ccctctccca gatgctgtcc atgggcttct ctgatgaagg cggctggctc accaggctcc
     1801 tgcagaccaa gaactatgac atcggagcgg ctctggacac catccagtat tcaaagcatc
     1861 ccccgccgtt gtgagaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct
     1921 agataactga tcataatcag ccataccaca tt
//
