LOCUS       sequence-87289-e        6038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6038)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             2445..2453
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      541 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga agtgctggga gcgcaagcgg aagcggaggc
      781 tccggtgagg aaacagctat atgggaacaa catacagtga cgcttcacag ggctcctgga
      841 tttggatttg gaattgcaat atctggtgga cgagataatc ctcattttca gagtggggaa
      901 acgtcaatag tgatttcaga tgtgctgaaa ggaggaccag ctgaaggaca gctacaggaa
      961 aatgaccgag ttgcaatggt taacggagtt tcaatggata atgttgaaca tgcttttgct
     1021 gttcagcaac taaggaaaag tgggaaaaat gcaaaaatta caattagaag gaagaagaaa
     1081 gttcaaatac cagtaagtcg tcctgatcct gaaccagtat ctgataatga agaagatagt
     1141 tatgatgagg aaatacatga tccaagaagt ggccggagtg gtgtggttaa cagaaggagt
     1201 gagaagattt ggccgaggga tagaagtgca agtagagaga ggagcttgtc cccgcggtca
     1261 gacaggcggt cagtggcttc cagccagcct gctaaaccta ctaaagtcac actggtgaaa
     1321 tcccggaaaa atgaagaata tggtcttcga ttggcaagcc atatatttgt taaggaaatt
     1381 tcacaagata gtttggcagc aagagatggc aatattcaag aaggtgatgt tgtattgaag
     1441 ataaatggta ctgtgacaga aaatatgtca ttgacagatg caaagacatt gatagaaagg
     1501 tctaaaggca aattaaaaat ggtagttcaa agagatgaac gggctacgct attgaatgtc
     1561 cctgatcttt ctgacagcat ccactctgct aatgcctctg agagagacga catttcagaa
     1621 attcagtcac tggcatcaga tcattctggt cgatcacacg ataggcctcc ccgccgcagc
     1681 cggtcacgat ctcctgacca gcggtcagag ccttctgatc attccaggca ctcgccgcag
     1741 cagccaagca atggcagtct gcggagtaga gatgaagaga gaatttctaa acctggggct
     1801 gtctcaactc ctgtaaagca tgctgatgat cacacaccta aaacagtgga agaagttaca
     1861 gttgaaagaa atgagaaaca aacaccttct cttccagaac caaagcctgt gtatgcccaa
     1921 gttggcaacc agatgtggat ttacctgtca gtccatctga tggtgtccta cctaattcaa
     1981 ctcatgaaga tgggatttct tcggcccagc atgaaattgg taaaattcag aaaaggagat
     2041 agtgtgggtt tgcggctggc tggtggaaat gatgttggaa tatttgtagc tggcgttcta
     2101 gaagatagcc ctgcagccaa ggaaggctta gaggaaggtg atcaaattct cagggtaaac
     2161 aacgtagatt ttacaaatat cataagagaa gaagccgtcc ttttcctgct tgacctccct
     2221 aaaggagaag aagtgaccat attggctcag aagaagaagg atgtttatcg tcgcattgta
     2281 gaatcagatg taggagattc tttctatatt agaacccatt ttgaatatga aaaggaatct
     2341 ccctatggac ttagttttaa caaaggagag gtgttccgtg ctgtggatac cttgtacaat
     2401 ggaaaactgg gctcttggct tgctattcga attggtaaaa atcataagga ggtagaacga
     2461 ggcatcatcc ctaataagaa cagagctgag cagctcgcca gtgtacagta tacacttcca
     2521 aaaacagcag gcggagaccg tgctgacttc tggagattca gaggtcttcg cagctccaag
     2581 agaaatcttc gaaaaagcag agaggatttg tccgctcagc ctgttcaaac aaagtttcca
     2641 gcttatgaaa gagtggttct tcgagaagct ggatttctga ggcctgtaac catttttgga
     2701 ccaatagctg atgttgccag agaaaagctg gcaagagaag aaccagatat ttatcaaatt
     2761 gcaaagagtg aaccacgaga cgctggaact gaccaacgta gctctggcta tattcgcctg
     2821 catacaataa agcaaatcat agatcaagac aaacatgctt tattagatgt aacaccaaat
     2881 gcagttgatc gtcttaacta tgcccagtgg tatccaattg ttgtatttct taaccctgat
     2941 tctaagcaag gagtaaaaac aatgagaatg aggttatgtc cagaatctcg gaaaagtgcc
     3001 aggaagttat acgagcgatc tcataaactt gctaaaaata atcaccatct ttttacaact
     3061 acaattaact taaattcaat gaatgatggt tggtatggtg cgctgaaaga agcagttcaa
     3121 caacagcaaa accagctggt atgggtttcc gagggaaagg cggatggtgc tacaagtgat
     3181 gaccttgatt tgcatgatga tcgtctgtcc tacctgtcag ctccaggtag tgaatactca
     3241 atgtatagca cggacagtag acacacttct gactatgaag acacagacac agaaggcggg
     3301 gcctacactg atcaagaact agatgaaact cttaatgatg aggttgggac tccaccggag
     3361 tctgccatta cacggtcctc tgagcctgta agagaggact cctctggaat gcatcatgaa
     3421 aaccaaacat atcctcctta ctcaccacaa gcgcagccac aaccaattca tagaatagac
     3481 tcccctggat ttaagccagc ctctcaacag aaagcagaag cttcatctcc agtcccttac
     3541 ctttcgcctg aaacaaaccc agcatcatca acctctgctg ttaatcataa tgtaaattta
     3601 actaatgtca gactggagga gcccacccca gctccttcca cctcttactc accacaagct
     3661 gattctttaa gaacaccaag tactgaggca gctcacataa tgctaagaga tcaagaacca
     3721 tcattgtcgt cgcatgtaga tccaacaaag gtgtatagaa aggacccata tcccgaggaa
     3781 atgatgaggc agaaccatgt tttgaaacag ccagccgtta gtcacccagg gcacaggcca
     3841 gacaaagagc ctaatctgac ctatgaaccc caactcccat acgtagagaa acaagccagc
     3901 agagacctgg agcagcccac atacagatac gagtcctcaa gctatacgga ccagttttct
     3961 cgaaactatg aacatcgtct gcgatacgaa gatcgcgtcc ccatgtatga agaacagtgg
     4021 tcatattatg atgacaaaca gccctaccca tctcggccac cttttgataa tcagcactct
     4081 caagaccttg actccagaca gcatcccgaa gagtcctcag aacgagggta ctttccacgt
     4141 tttgaagagc cagcccctct gtcttacgac agcagaccac gttacgaaca ggcacctaga
     4201 gcatccgccc tgcggcacga agagcagcca gctcctgggt atgacacaca tggtagactc
     4261 agaccggaag cccagcccca cccttcagca gggcccaagc ctgcagagtc caagcagtat
     4321 tttgagcaat attcacgcag ttacgagcaa gtaccacccc aaggatttac ctctagagca
     4381 ggtcattttg agcctctcca tggtgctgca gctgtccctc cgctgatacc ttcatctcag
     4441 cataagccag aagctctgcc ttcaaacacc aaaccactgc ctccaccccc aactcaaacc
     4501 gaagaagagg aagatccagc aatgaagcca cagtctgtac tcaccagagt taagatgttt
     4561 gaaaacaaaa ggtctgcatc cttagagacc aagaaggatg taaatgacac tggcagtttt
     4621 aagcctccag aagtagcatc taaaccttca ggtgctccca tcattggtcc caaacccact
     4681 tctcagaatc aattcagtga acatgacaaa actctgtaca ggattccaga acctcaaaaa
     4741 cctcaactga agccacctga agatattgtt cggtccaatc attatgaccc tgaagaagat
     4801 gaagaatatt atcgaaaaca gctgtcatac tttgaccgaa gaagttttga gaataagcct
     4861 cctgcacaca ttgctgccag ccatctctcc gagcctgcaa agccagctca ttctcagaat
     4921 caatcaaatt tttctagtta ttcttcaaag ggaaagcctc ctgaagctga tggtgtggat
     4981 agatcatttg gcgagaaacg ctatgaaccc atccaggcca ctccccctcc tcctccattg
     5041 ccctcgcagt atgcccagcc atctcagcct gtcaccagcg cgtctctcca catacattct
     5101 aagggagcac atggtgaagg taattcagtg tcattggatt ttcagaattc cttagtgtcc
     5161 aaaccagacc cacctccatc tcagaataag ccagcaactt tcagaccacc aaaccgagaa
     5221 gatactgctc aggcagcttt ctatccccag aaaagttttc cagataaagc cccagttaat
     5281 ggaactgaac agactcagaa aacagtcact ccagcataca atcgattcac accaaaacca
     5341 tatacaagtt ctgcccgacc atttgaacgc aagtttgaaa gtcctaaatt caatcacaat
     5401 cttctgccaa gtgaaactgc acataaacct gacttgtctt caaaaactcc cacttctcca
     5461 aaaactcttg tgaaatcgca cagtttggca cagcctcctg agtttgacag tggagttgaa
     5521 actttctcta tccatgcaga gaagcctaaa tatcaaataa ataatatcag cacagtgcct
     5581 aaagctattc ctgtgagtcc ttcagctgtg gaagaggatg aagatgaaga tggtcatact
     5641 gtggtggcca cagcccgagg catatttaac agcaatgggg gcgtgctgag ttccatagaa
     5701 actggtgtta gtataattat ccctcaagga gccattcccg aaggagttga gcaggaaatc
     5761 tatttcaagg tctgccggga caacagcatc cttccacctt tagataaaga gaaaggtgaa
     5821 acactgctga gtcctttggt gatgtgtggt ccccatggcc tcaagttcct gaagcctgtg
     5881 gagctgcgct taccacactg tgatcctaaa acctggcaaa acaagtgtct tcccggagat
     5941 ccaaattatc tcgttggagc aaactgtgtt tctgtcctta ttgaccactt ttaaggatcc
     6001 accggatcta gataactgat cataatcagc cataccac
//
