LOCUS       sequence-87294-e        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..744
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggggcaatcg
       61 aagcatggac taaaggagga gatgacggtg aagtaccaca tggaaggttg cgttaatggt
      121 cacaaatttg tcattactgg agagggcatt ggaaaccctt ttaagggtaa gcaaaccgca
      181 aatttgtgtg tgatagaagg aggcccgctg ccgttctcgg aggacatttt aagtccgggt
      241 tttaaatatg gtgaccggat tttcacagag tacccacagg atattgtaga ttacttcaag
      301 aactcatgtc cagcgggcta tacgtgggaa aggagctacc tctttgagga cggagcggtc
      361 tgtcgatgca acgtggacat aacagtctct gaaaaggaga actgcatcta tcacaaaagt
      421 atcttcagag gggtgaattt tcccgccgac ggccccgtaa tgaagaaaat gaccactaat
      481 tgggaagcta gtaccgagaa aattgtgcct gttccaaagc aagggatatt aaagggaaag
      541 gtcaaaatgt gcctgttgct gaaggatggc ggtcgttatc attgccagtt tgatacggta
      601 tataaagcta aatcagtgcc ctccaagatg ccagaatggc attttataca gcataagctc
      661 cttagggagg accgctctga tgctaaaaac cagaaatggc aattaacaga acatgcaatc
      721 gcaggcatgg acgagctgta caagtccgga ctcagatctg gcagcggtgg aggcagcgca
      781 tccggcggaa gcggaagcga tgatgatatc gccgcgctcg tcgtcgacaa cggctccggc
      841 atgtgcaagg ccggcttcgc gggcgacgat gccccccggg ccgtcttccc ctccatcgtg
      901 gggcgcccca ggcaccaggg cgtgatggtg ggcatgggtc agaaggattc ctatgtgggc
      961 gacgaggccc agagcaagag aggcatcctc accctgaagt accccatcga gcacggcatc
     1021 gtcaccaact gggacgacat ggagaaaatc tggcaccaca ccttctacaa tgagctgcgt
     1081 gtggctcccg aggagcaccc cgtgctgctg accgaggccc ccctgaaccc caaggccaac
     1141 cgcgagaaga tgacccagat catgtttgag accttcaaca ccccagccat gtacgttgct
     1201 atccaggctg tgctatccct gtacgcctct ggccgtacca ctggcatcgt gatggactcc
     1261 ggtgacgggg tcacccacac tgtgcccatc tacgaggggt atgccctccc ccatgccatc
     1321 ctgcgtctgg acctggctgg ccgggacctg actgactacc tcatgaagat cctcaccgag
     1381 cgcggctaca gcttcaccac cacggccgag cgggaaatcg tgcgtgacat taaggagaag
     1441 ctgtgctacg tcgccctgga cttcgagcaa gagatggcca cggctgcttc cagctcctcc
     1501 ctggagaaga gctacgagct gcctgacggc caggtcatca ccattggcaa tgagcggttc
     1561 cgctgccctg aggcactctt ccagccttcc ttcctgggca tggagtcctg tggcatccac
     1621 gaaactacct tcaactccat catgaagtgt gacgtggaca tccgcaaaga cctgtacgcc
     1681 aacacagtgc tgtctggcgg caccaccatg taccctggca ttgccgacag gatgcagaag
     1741 gagatcactg ccctggcacc cagcacaatg aagatcaaga tcattgctcc tcctgagcgc
     1801 aagtactccg tgtggatcgg cggctccatc ctggcctcgc tgtccacctt ccagcagatg
     1861 tggatcagca agcaggagta tgacgagtcc ggcccctcca tcgtccaccg caaatgcttc
     1921 tagggatcca ccggatctag ataactgatc ataatcagcc ataccacatt gtagag
//
