LOCUS       sequence-87295-e        1185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..734
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     CDS             765..1142
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 cgctagcgct accggtcgcc accatggtga gcaagggcga ggggcaatcg aagcatggac
       61 taaaggagga gatgacggtg aagtaccaca tggaaggttg cgttaatggt cacaaatttg
      121 tcattactgg agagggcatt ggaaaccctt ttaagggtaa gcaaaccgca aatttgtgtg
      181 tgatagaagg aggcccgctg ccgttctcgg aggacatttt aagtccgggt tttaaatatg
      241 gtgaccggat tttcacagag tacccacagg atattgtaga ttacttcaag aactcatgtc
      301 cagcgggcta tacgtgggaa aggagctacc tctttgagga cggagcggtc tgtcgatgca
      361 acgtggacat aacagtctct gaaaaggaga actgcatcta tcacaaaagt atcttcagag
      421 gggtgaattt tcccgccgac ggccccgtaa tgaagaaaat gaccactaat tgggaagcta
      481 gtaccgagaa aattgtgcct gttccaaagc aagggatatt aaagggaaag gtcaaaatgt
      541 gcctgttgct gaaggatggc ggtcgttatc attgccagtt tgatacggta tataaagcta
      601 aatcagtgcc ctccaagatg ccagaatggc attttataca gcataagctc cttagggagg
      661 accgctctga tgctaaaaac cagaaatggc aattaacaga acatgcaatc gcaggcatgg
      721 acgagctgta caagtccgga ctcagatctg gaggctccgg aggcccagag ccagcgaagt
      781 ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg
      841 gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga
      901 agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg
      961 tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc
     1021 gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt
     1081 tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagt
     1141 aaggatccac cggatctaga taactgatca taatcagcca tacca
//
