LOCUS       sequence-87298-e        1191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 492, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1191)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAzami Green-H2B
FEATURES             Location/Qualifiers
     source          1..1191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..454
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             473..1153
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             473..1153
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt
       61 cgacggtacc gccaccatgc cagagccagc gaagtctgct cccgccccga aaaagggctc
      121 caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa
      181 ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat
      241 ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc
      301 aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat
      361 ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg
      421 tactaaggcc atcaccaagt acaccagcgc taaggatcca ccggtcgcca ccatggtgag
      481 cgtgatcaag cccgagatga agatcaagct gtgcatgagg ggcaccgtga acggccacaa
      541 cttcgtgatc gagggcgagg gcaagggcaa cccctacgag ggcacccaga tcctggacct
      601 gaacgtgacc gagggcgccc ccctgccctt cgcctacgac atcctgacca ccgtgttcca
      661 gtacggcaac agggccttca ccaagtaccc cgccgacatc caggactact tcaagcagac
      721 cttccccgag ggctaccact gggagaggag catgacctac gaggaccagg gcatctgcac
      781 cgccaccagc aacatcagca tgaggggcga ctgcttcttc tacgacatca ggttcgacgg
      841 caccaacttc ccccccaacg gccccgtgat gcagaagaag accctgaagt gggagcccag
      901 caccgagaag atgtacgtgg aggacggcgt gctgaagggc gacgtgaaca tgaggctgct
      961 gctggagggc ggcggccact acaggtgcga cttcaagacc acctacaagg ccaagaagga
     1021 ggtgaggctg cccgacgccc acaagatcga ccacaggatc gagatcctga agcacgacaa
     1081 ggactacaac aaggtgaagc tgtacgagaa cgccgtggcc aggtactcca tgctgcccag
     1141 ccaggccaag taagcggccg cgactctaga tcataatcag ccataccaca t
//
