LOCUS       sequence-87299-e        4008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 492, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4008)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAzami Green-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..702
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             25..702
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
ORIGIN
        1 ccgctagcgc taccggtcgc caccatggtg agcgtgatca agcccgagat gaagatcaag
       61 ctgtgcatga ggggcaccgt gaacggccac aacttcgtga tcgagggcga gggcaagggc
      121 aacccctacg agggcaccca gatcctggac ctgaacgtga ccgagggcgc ccccctgccc
      181 ttcgcctacg acatcctgac caccgtgttc cagtacggca acagggcctt caccaagtac
      241 cccgccgaca tccaggacta cttcaagcag accttccccg agggctacca ctgggagagg
      301 agcatgacct acgaggacca gggcatctgc accgccacca gcaacatcag catgaggggc
      361 gactgcttct tctacgacat caggttcgac ggcaccaact tcccccccaa cggccccgtg
      421 atgcagaaga agaccctgaa gtgggagccc agcaccgaga agatgtacgt ggaggacggc
      481 gtgctgaagg gcgacgtgaa catgaggctg ctgctggagg gcggcggcca ctacaggtgc
      541 gacttcaaga ccacctacaa ggccaagaag gaggtgaggc tgcccgacgc ccacaagatc
      601 gaccacagga tcgagatcct gaagcacgac aaggactaca acaaggtgaa gctgtacgag
      661 aacgccgtgg ccaggtactc catgctgccc agccaggcca agtccggact cagatctcga
      721 gctcaagctt cgaattcggg aaactcgaga acaaagcttg aaaacggccg catgccagtg
      781 tttcatacgc gcacgatcga gagcatcctg gagccggtgg cacagcagat ctcccacctg
      841 gtgataatgc acgaggaggg cgaggtggac ggcaaagcca ttcctgacct caccgcgccc
      901 gtggccgccg tgcaggcggc cgtcagcaac ctcgtccggg ttggaaaaga gactgttcaa
      961 accactgagg atcagatttt gaagagagat atgccaccag catttattaa ggttgagaat
     1021 gcttgcacca agcttgtcca ggcagctcag atgcttcagt cagaccctta ctcagtgcct
     1081 gctcgagatt atctaattga tgggtcaagg ggcatcctct ctggaacatc agacctgctc
     1141 cttaccttcg atgaggctga ggtccgtaaa attattagag tttgcaaagg aattttggaa
     1201 tatcttacag tggcagaggt ggtggagact atggaagatt tggtcactta cacaaagaat
     1261 cttgggccag gaatgactaa gatggccaag atgattgacg agagacagca ggagctcact
     1321 caccaggagc accgagtgat gttggtgaac tcgatgaaca ccgtgaaaga gttgctgcca
     1381 gttctcattt cagctatgaa gatttttgta acaactaaaa actcaaaaaa ccaaggcata
     1441 gaggaagctt taaaaaatcg caattttact gtagaaaaaa tgagtgctga aattaatgag
     1501 ataattcgtg tgttacaact cacctcttgg gatgaagatg cctgggccag caaggacact
     1561 gaagccatga agagagcatt ggcctccata gactccaaac tgaaccaggc caaaggttgg
     1621 ctccgtgacc ctagtgcctc cccaggggat gctggtgagc aggccatcag acagatctta
     1681 gatgaagctg gaaaagttgg tgaactctgt gcaggcaaag aacgcaggga gattctggga
     1741 acttgcaaaa tgctagggca gatgactgat caagtggctg acctccgtgc cagaggacaa
     1801 ggatcctcac cggtggccat gcagaaagct cagcaggtat ctcagggtct ggatgtgctc
     1861 acagcaaaag tggaaaatgc agctcgcaag ctggaagcca tgaccaactc aaagcagagc
     1921 attgcaaaga agatcgatgc tgctcagaac tggcttgcag atccaaatgg tggaccggaa
     1981 ggagaagagc agattcgagg tgctttggct gaagctcgga aaatagcaga attatgtgat
     2041 gatcctaaag aaagagatga cattctacgt tcccttgggg aaatatctgc tctgacttct
     2101 aaattagcag atctacgaag acaggggaaa ggagattctc cagaggctcg agccttggcc
     2161 aaacaggtgg ccacggccct gcagaacctg cagaccaaaa ccaaccgggc tgtggccaac
     2221 agcagaccgg ccaaagcagc tgtacacctt gagggcaaga ttgagcaagc acagcggtgg
     2281 attgataatc ccacagtgga tgaccgtgga gtcggtcagg ctgccatccg ggggcttgtg
     2341 gccgaagggc atcgtctggc taatgttatg atggggcctt atcggcaaga tcttctcgcc
     2401 aagtgtgacc gagtggacca gctgacagcc cagctggctg acctggctgc cagaggggaa
     2461 ggggagagtc ctcaggcacg agcacttgca tctcagctcc aagactcctt aaaggatcta
     2521 aaagctcgga tgcaggaggc catgactcag gaagtgtcag atgttttcag cgataccaca
     2581 actcccatca agctgttggc agtggcagcc acggcgcctc ctgatgcgcc taacagggaa
     2641 gaggtatttg atgagagggc agctaacttt gaaaaccatt caggaaagct tggtgctacg
     2701 gccgagaagg cggctgcggt tggtactgct aataaatcaa cagtggaagg cattcaggcc
     2761 tcagtgaaga cggcccgaga actcacaccc caggtggtct cggctgctcg tatcttactt
     2821 aggaaccctg gaaatcaagc tgcttatgaa cattttgaga ccatgaagaa ccagtggatc
     2881 gataatgttg aaaaaatgac agggctggtg gacgaagcca ttgataccaa atctctgttg
     2941 gatgcttcag aagaagcaat taaaaaagac ctggacaagt gcaaggtagc tatggccaac
     3001 attcagcctc agatgctggt tgctggggca accagtattg ctcgtcgggc caaccggatc
     3061 ctgctggtgg ctaagaggga ggtggagaat tccgaggatc ccaagttccg tgaggctgtg
     3121 aaagctgcct ctgatgaatt gagcaaaacc atctccccga tggtgatgga tgcaaaagct
     3181 gtggctggaa acatttccga ccctggactg caaaagagct tcctggactc aggatatcgg
     3241 atcctgggag ctgtggccaa ggtcagagaa gccttccaac ctcaggagcc tgacttcccg
     3301 ccgcctccac cagaccttga acaactccga ctaacagatg agcttgctcc tcccaaacca
     3361 cctctgcctg aaggtgaggt ccctccacct aggcctccac caccagagga aaaggatgaa
     3421 gagttccctg agcagaaggc cggggaggtg attaaccagc caatgatgat ggctgccaga
     3481 cagctccatg atgaagctcg caaatggtcc agcaagggca atgacatcat tgcagcagcc
     3541 aagcgcatgg ctctgctgat ggctgagatg tctcggctgg taagaggggg cagtggtacc
     3601 aagcgggcac tcattcagtg tgccaaggac atcgccaagg cctcagatga ggtgactcgg
     3661 ttggccaagg aggttgccaa gcagtgcaca gataaacgga ttagaaccaa cctcttacag
     3721 gtatgtgagc gaatcccaac cataagcacc cagctcaaaa tcctgtccac agtgaaggcc
     3781 accatgctgg gccggaccaa catcagtgat gaggagtctg agcaggccac agagatgctg
     3841 gttcacaatg cccagaacct catgcagtct gtgaaggaga ctgtgcggga agctgaagct
     3901 gcttcaatca aaattcgaac agatgctgga tttacactgc gctgggttag aaagactccc
     3961 tggtaccagt aggcggccgc gactctagat cataatcagc cataccac
//
