LOCUS       sequence-87313-e        1950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: EGFP-Actin-7
FEATURES             Location/Qualifiers
     source          1..1950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      241 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctatggatga
      781 tgatatcgcc gcgctcgtcg tcgacaacgg ctccggcatg tgcaaggccg gcttcgcggg
      841 cgacgatgcc ccccgggccg tcttcccctc catcgtgggg cgccccaggc accagggcgt
      901 gatggtgggc atgggtcaga aggattccta tgtgggcgac gaggcccaga gcaagagagg
      961 catcctcacc ctgaagtacc ccatcgagca cggcatcgtc accaactggg acgacatgga
     1021 gaaaatctgg caccacacct tctacaatga gctgcgtgtg gctcccgagg agcaccccgt
     1081 gctgctgacc gaggcccccc tgaaccccaa ggccaaccgc gagaagatga cccagatcat
     1141 gtttgagacc ttcaacaccc cagccatgta cgttgctatc caggctgtgc tatccctgta
     1201 cgcctctggc cgtaccactg gcatcgtgat ggactccggt gacggggtca cccacactgt
     1261 gcccatctac gaggggtatg ccctccccca tgccatcctg cgtctggacc tggctggccg
     1321 ggacctgact gactacctca tgaagatcct caccgagcgc ggctacagct tcaccaccac
     1381 ggccgagcgg gaaatcgtgc gtgacattaa ggagaagctg tgctacgtcg ccctggactt
     1441 cgagcaagag atggccacgg ctgcttccag ctcctccctg gagaagagct acgagctgcc
     1501 tgacggccag gtcatcacca ttggcaatga gcggttccgc tgccctgagg cactcttcca
     1561 gccttccttc ctgggcatgg agtcctgtgg catccacgaa actaccttca actccatcat
     1621 gaagtgtgac gtggacatcc gcaaagacct gtacgccaac acagtgctgt ctggcggcac
     1681 caccatgtac cctggcattg ccgacaggat gcagaaggag atcactgccc tggcacccag
     1741 cacaatgaag atcaagatca ttgctcctcc tgagcgcaag tactccgtgt ggatcggcgg
     1801 ctccatcctg gcctcgctgt ccaccttcca gcagatgtgg atcagcaagc aggagtatga
     1861 cgagtccggc ccctccatcg tccaccgcaa atgcttctag ggatccaccg gatctagata
     1921 actgatcata atcagccata ccacattgag
//
