LOCUS       sequence-87315-e        4060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4060)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP-Alpha-5-Integrin-10
FEATURES             Location/Qualifiers
     source          1..4060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3295..3304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3301..3303,3304..3306,3307..4020)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3301..3303,3304..3306,3307..4017)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3346..3367)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3607..3626)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3954..3975
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttg catgcctgca
       61 ggtcgacctg cagcccaagc tcgatgaatt cgagctcggt acccggggat cctctagacc
      121 gctatgggga gccggacgcc agagtcccct ctccacgccg tgcagctgcg ctggggcccc
      181 cggcgccgac ccccgctgct gccgctgctg ttgctgctgc tgccgccgcc acccagggtc
      241 gggggcttca acttagacgc ggaggcccca gcagtactct cggggccccc gggctccttc
      301 ttcggattct cagtggagtt ttaccggccg ggaacagacg gggtcagtgt gctggtggga
      361 gcacccaagg ctaataccag ccagccagga gtgctgcagg gtggtgctgt ctacctctgt
      421 ccttggggtg ccagccccac acagtgcacc cccattgaat ttgacagcaa aggctctcgg
      481 ctcctggagt cctcactgtc cagctcagag ggagaggagc ctgtggagta caagtccttg
      541 cagtggttcg gggcaacagt tcgagcccat ggctcctcca tcttggcatg cgctccactg
      601 tacagctggc gcacagagaa ggagccactg agcgaccccg tgggcacctg ctacctctcc
      661 acagataact tcacccgaat tctggagtat gcaccctgcc gctcagattt cagctgggca
      721 gcaggacagg gttactgcca aggaggcttc agtgccgagt tcaccaagac tggccgtgtg
      781 gttttaggtg gaccaggaag ctatttctgg caaggccaga tcctgtctgc cactcaggag
      841 cagattgcag aatcttatta ccccgagtac ctgatcaacc tggttcaggg gcagctgcag
      901 actcgccagg ccagttccat ctatgatgac agctacctag gatactctgt ggctgttggt
      961 gaattcagtg gtgatgacac agaagacttt gttgctggtg tgcccaaagg gaacctcact
     1021 tacggctatg tcaccatcct taatggctca gacattcgat ccctctacaa cttctcaggg
     1081 gaacagatgg cctcctactt tggctatgca gtggccgcca cagacgtcaa tggggacggg
     1141 ctggatgact tgctggtggg ggcacccctg ctcatggatc ggacccctga cgggcggcct
     1201 caggaggtgg gcagggtcta cgtctacctg cagcacccag ccggcataga gcccacgccc
     1261 acccttaccc tcactggcca tgatgagttt ggccgatttg gcagctcctt gacccccctg
     1321 ggggacctgg accaggatgg ctacaatgat gtggccatcg gggctccctt tggtggggag
     1381 acccagcagg gagtagtgtt tgtatttcct gggggcccag gagggctggg ctctaagcct
     1441 tcccaggttc tgcagcccct gtgggcagcc agccacaccc cagacttctt tggctctgcc
     1501 cttcgaggag gccgagacct ggatggcaat ggatatcctg atctgattgt ggggtccttt
     1561 ggtgtggaca aggctgtggt atacaggggc cgccccatcg tgtccgctag tgcctccctc
     1621 accatcttcc ccgccatgtt caacccagag gagcggagct gcagcttaga ggggaaccct
     1681 gtggcctgca tcaaccttag cttctgcctc aatgcttctg gaaaacacgt tgctgactcc
     1741 attggtttca cagtggaact tcagctggac tggcagaagc agaagggagg ggtacggcgg
     1801 gcactgttcc tggcctccag gcaggcaacc ctgacccaga ccctgctcat ccagaatggg
     1861 gctcgagagg attgcagaga gatgaagatc tacctcagga acgagtcaga atttcgagac
     1921 aaactctcgc cgattcacat cgctctcaac ttctccttgg acccccaagc cccagtggac
     1981 agccacggcc tcaggccagc cctacattat cagagcaaga gccggataga ggacaaggct
     2041 cagatcttgc tggactgtgg agaagacaac atctgtgtgc ctgacctgca gctggaagtg
     2101 tttggggagc agaaccatgt gtacctgggt gacaagaatg ccctgaacct cactttccat
     2161 gcccagaatg tgggtgaggg tggcgcctat gaggctgagc ttcgggtcac cgcccctcca
     2221 gaggctgagt actcaggact cgtcagacac ccagggaact tctccagcct gagctgtgac
     2281 tactttgccg tgaaccagag ccgcctgctg gtgtgtgacc tgggcaaccc catgaaggca
     2341 ggagccagtc tgtggggtgg ccttcggttt acagtccctc atctccggga cactaagaaa
     2401 accatccagt ttgacttcca gatcctcagc aagaatctca acaactcgca aagcgacgtg
     2461 gtttcctttc ggctctccgt ggaggctcag gcccaggtca ccctgaacgg tgtctccaag
     2521 cctgaggcag tgctattccc agtaagcgac tggcatcccc gagaccagcc tcagaaggag
     2581 gaggacctgg gacctgctgt ccaccatgtc tatgagctca tcaaccaagg ccccagctcc
     2641 attagccagg gtgtgctgga actcagctgt ccccaggctc tggaaggtca gcagctccta
     2701 tatgtgacca gagttacggg actcaactgc accaccaatc accccattaa cccaaagggc
     2761 ctggagttgg atcccgaggg ttccctgcac caccagcaaa aacgggaagc tccaagccgc
     2821 agctctgctt cctcgggacc tcagatcctg aaatgcccgg aggctgagtg tttcaggctg
     2881 cgctgtgagc tcgggcccct gcaccaacaa gagagccaaa gtctgcagtt gcatttccga
     2941 gtctgggcca agactttctt gcagcgggag caccagccat ttagcctgca gtgtgaggct
     3001 gtgtacaaag ccctgaagat gccctaccga atcctgcctc ggcagctgcc ccaaaaagag
     3061 cgtcaggtgg ccacagctgt gcaatggacc aaggcagaag gcagctatgg cgtcccactg
     3121 tggatcatca tcctagccat cctgtttggc ctcctgctcc taggtctact catctacatc
     3181 ctctacaagc ttggattctt caaacgctcc ctcccatatg gcaccgccat ggaaaaagct
     3241 cagctcaagc ctccagccac ctctgatgcc ggtaccgcgg gcccgggatc catcgccacc
     3301 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     3361 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     3421 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3481 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     3541 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3601 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3661 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3721 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     3781 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     3841 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3901 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3961 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     4021 agcggccgcg actctagatc ataatcagcc ataccacatt
//
