LOCUS       sequence-87334-e        1462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Fang Z, Takizawa N, Wilson KA, Smith TC, Delprato A, Davidson MW, 
            Lambright DG, Luna EJ
  TITLE     The membrane-associated protein, supervillin, accelerates 
            F-actin-dependent rapid integrin recycling and cell motility.
  JOURNAL   Traffic. 2010 Jun;11(6):782-99. doi: 
            10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17.
  PUBMED    20331534
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01062.x"; journalName: "Traffic"; date: 
            "2010-06"; volume: "11"; issue: "6"; pages: "782-99"
COMMENT     Sequence Label: EGFP-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      241 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      481 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagccatg gctagtcgag
      781 gcgcaacaag acccaacggg ccaaatactg gaaataaaat atgccagttc aaactagtac
      841 ttctgggaga gtctgctgtt ggcaaatcaa gcctagtgct tcgttttgtg aagggccaat
      901 ttcatgaatt tcaagagagt accatagggg ctgcttttct aacccaaact gtgtgtcttg
      961 atgatacaac agtaaagttt gaaatatggg atacagctgg tcaagaacga taccatagct
     1021 tagcaccaat gtactacaga ggagcacaag cagccatagt tgtatatgat atcacaaatg
     1081 aggagtcctt tgccagagcc aaaaactggg ttaaagaact tcagaggcaa gccagtccta
     1141 acattgtaat agctttatca ggaaacaagg ctgatcttgc aaataaaaga gctgtcgatt
     1201 tccaggaagc acagtcctat gcagatgaca acagtttatt attcatggag acatcagcta
     1261 aaacatcgat gaatgtaaat gaaatattca tggcaatagc taaaaagttg ccaaagaacg
     1321 aaccacagaa tccaggagca aattctgcca gaggaagagg agtagacctt actgaaccca
     1381 cgcagccaac caggaatcag tgttgtagta actaaggatc caccggatct agataactga
     1441 tcataatcag ccataccaca tt
//
