LOCUS       sequence-87335-e        1443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Fang Z, Takizawa N, Wilson KA, Smith TC, Delprato A, Davidson MW, 
            Lambright DG, Luna EJ
  TITLE     The membrane-associated protein, supervillin, accelerates 
            F-actin-dependent rapid integrin recycling and cell motility.
  JOURNAL   Traffic. 2010 Jun;11(6):782-99. doi: 
            10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17.
  PUBMED    20331534
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01062.x"; journalName: "Traffic"; date: 
            "2010-06"; volume: "11"; issue: "6"; pages: "782-99"
COMMENT     Sequence Label: EGFP-Rab7a-7
FEATURES             Location/Qualifiers
     source          1..1443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      241 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ccatgacctc
      781 taggaagaaa gtgttgctga aggttatcat cctgggagat tctggagtcg ggaagacatc
      841 actcatgaac cagtatgtga ataagaaatt cagcaatcag tacaaagcca caataggagc
      901 tgactttctg accaaggagg tgatggtgga tgacaggcta gtcacaatgc agatatggga
      961 cacagcagga caggaacggt tccagtctct cggtgtggcc ttctacagag gtgcagactg
     1021 ctgcgttctg gtatttgatg tgactgcccc caacacattc aaaaccctag atagctggag
     1081 agatgagttt ctcatccagg ccagtccccg agatcctgaa agcttcccat ttgttgtgtt
     1141 gggaaacaag attgacctcg aaaacagaca agtggccaca aagcgggcac aggcctggtg
     1201 ctacagcaaa aacaacattc cctactttga gaccagtgcc aaggaggcca tcaacgtgga
     1261 gcaggcgttc cagacgattg cacggaatgc acttaagcag gaaacggagg tggagctgta
     1321 caacgaattt cctgaaccta tcaaactgga caagaatgac cgggccaagg cctcggcaga
     1381 aagctgcagt tgctgaggat ccaccggatc tagataactg atcataatca gccataccac
     1441 att
//
