LOCUS       sequence-87341-e        2172 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin Head Domain, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2172)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP-Talin-H-18
FEATURES             Location/Qualifiers
     source          1..2172
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      800..809
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      241 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagctag
      781 cttcgaattc gcagaagctg ccaccatggt tgcgctttcg ctgaagatta gcattgggaa
      841 tgtggtgaag acgatgcaat ttgagccatc taccatggtg tacgacgcct gccgcatgat
      901 tcgtgagcgg atcccagagg ccctggctgg ccctcccaac gactttgggc tctttctgtc
      961 agatgatgac cccaaaaaag gcatctggct agaggctggg aaagctttgg actactacat
     1021 gctccgaaat ggggacacca tggagtacag aaaaaagcag agacccctga agatccggat
     1081 gttagacgga acggtgaaga ctatcatggt ggatgactcc aagaccgtca cagacatgct
     1141 catgaccatt tgtgcccgaa ttggtatcac caaccatgat gagtattcac tggttcgaga
     1201 gctgatggaa gaaaagaaag atgaggggac agggaccctg agaaaagaca agaccctact
     1261 gcgagatgaa aagaagatgg agaagctaaa gcagaagttg cacacagacg atgagttgaa
     1321 ctggctggac catgggcgga cactgaggga acagggagtg gaagagcatg agacgttgct
     1381 gctgcggaga aagttcttct actcagacca gaatgtggat tcccgagacc ctgtacagct
     1441 gaaccttctc tatgtgcagg cacgagatga catcctgaat ggctcccatc ctgtctcctt
     1501 tgacaaggcc tgtgaatttg caggcttcca gtgccagatc cagtttggac ctcacaatga
     1561 acagaagcac aaggctggct tccttgacct gaaggacttc ctgcccaagg agtatgtgaa
     1621 gcagaaggga gagcgtaaga tctttcaggc acacaagaat tgtgggcaga tgagtgaaat
     1681 tgaggccaag gtacgctacg tgaagctggc ccgttccctc aagacttacg gtgtctcctt
     1741 cttcctagtc aaggaaaaga tgaaggggaa gaataaactg gtgcccaggc tgttgggcat
     1801 cactaaggag tgtgtgatgc gtgtggatga gaagaccaag gaggtgattc aggaatggag
     1861 cctcaccaac atcaaacgct gggctgcctc tcccaagagc tttactctgg actttggaga
     1921 ctatcaggat ggctactact cagtacagac gacagaaggc gagcagatcg cacagctcat
     1981 tgctggctac atagatatca tccttaagaa gaaaaaaagc aaggaccatt ttgggctgga
     2041 gggagatgaa gagtctacta tgttggagga ctcagtgtct cccaaaaagt caacagtcct
     2101 tcagtaagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
     2161 cagccatacc ac
//
