LOCUS       sequence-87356-e        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEGFP-H4-23
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    337..389
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      397..406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(403..405,406..408,409..1122)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(448..469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(709..728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1056..1077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagccgc caccatgtct ggccgcggca aaggcgggaa gggtcttggc
       61 aaaggcggcg ctaagcgcca ccgtaaagta ctgcgcgaca atatccaggg catcaccaag
      121 ccggccatcc ggcgccttgc tcgccgcggc ggcgtgaagc gcatctccgg cctcatctac
      181 gaggagactc gcggggtgct gaaggtgttc ctggagaacg tgatccggga cgccgtgacc
      241 tatacagagc acgccaagcg caagacggtc accgccatgg atgtggtcta cgcgctcaag
      301 cgccagggcc gcaccctcta cggtttcggt ggggatctcg agctcaagct tcgaattctg
      361 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      421 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      481 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      541 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      601 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      661 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      721 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      781 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      841 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      901 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      961 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1021 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1081 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     1141 tcataatcag ccataccac
//
