LOCUS       sequence-87360-e        2081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mEGFP-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(41..103,104..1261)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1316..1325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1322..1324,1325..1327,1328..2041)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1367..1388)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1628..1647)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1975..1996
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagcgccacc atggcggccc cgggcgcccg
       61 gcgtccgctg ctcctgttgc tgctggcagg ccttgcacac agcgccccag cactgttcga
      121 ggtgaaagac aacaacggca cagcgtgtat aatggccagc ttctctgcct cctttctgac
      181 cacctatgag gctggacatg tttctaaggt ctcgaatatg accctgccag cctctgcaga
      241 agtcctgaag aatagcagct cttgtggtga aaagaatgct tctgagccca ccctcgcaat
      301 cacctttgga gaaggatatt tactgaaact caccttcaca aaaaacacaa cacgttacag
      361 tgtccagcac atgtatttca catataacct gtcagacaca caattctttc ccaatgccag
      421 ctccaaaggg cccgacactg tggattccac aactgacatc aaggcagaca tcaacaaaac
      481 ataccgatgt gtcagcgaca tcagggtcta catgaagaat gtgaccattg tgctctggga
      541 cgctactatc caggcctacc tgccgagtag caacttcagc aaggaagaga cacgctgccc
      601 acaggatcaa ccttccccaa ctactgggcc acccagcccc tcaccaccac ttgtgcccac
      661 aaaccccagt gtgtccaagt acaatgtgac tggtgacaat ggaacctgcc tgctggcctc
      721 tatggcactg caactcaaca tcacctacat gaagaaggac aacacgactg tgaccagagc
      781 attcaacatc aacccaagtg acaaatatag tgggacttgc ggtgcccagt tggtgaccct
      841 gaaggtgggg aacaagagca gagtcctgga gctgcagttt gggatgaatg ccacttctag
      901 cctgtttttc ctgcaaggag ttcagttgaa catgactctt cctgatgcca tagagcccac
      961 gttcagcacc tccaactatt ccctgaaagc tcttcaggcc agtgtcggca actcatacaa
     1021 gtgcaacagt gaggagcaca tctttgtcag caaggcgctc gccctcaatg tcttcagcgt
     1081 gcaagtccag gctttcaggg tagaaagtga caggtttggg tctgtggaag agtgtgtaca
     1141 ggacggtaac aacatgctga tccccattgc tgtgggcggg gccctggcag ggctggtcct
     1201 catcgtcctc atcgcctacc tcatcggcag gaagaggagt cacgcgggct atcagaccat
     1261 ctcggaattc ggctccaccg gctccaccgg ctccaccggc gcggatccac cggtcgccac
     1321 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1381 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1441 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1501 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1561 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1621 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1681 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1741 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     1801 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     1861 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1921 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     1981 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2041 aagcggccgc gactctagat cataatcagc cataccacat t
//
