LOCUS       sequence-87362-e        1716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1716)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEGFP-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..49
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             77..97
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(110..112,113..115,116..826)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(155..176)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(416..435)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     763..784
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcca ccatggatcc aaaaaagaag agaaaggtag atccaaaaaa
       61 gaagagaaag gtagatccaa aaaagaagag aaaggtaccg gtcgccacca tggtgagcaa
      121 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      181 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      241 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      301 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      361 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      421 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      481 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      541 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      601 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      661 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      721 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      781 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gagaagggca
      841 agggcaaggg caagggccgg gccgcggcta cgcgtatcga tccatgttcg aggcgcgcct
      901 ggtccagggc tccatcctca agaaggtgtt ggaggcactc aaggacctca tcaacgaggc
      961 ctgctgggat attagctcca gcggtgtaaa cctgcagagc atggactcgt cccacgtctc
     1021 tttggtgcag ctcaccctgc ggtctgaggg cttcgacacc taccgctgcg accgcaacct
     1081 ggccatgggc gtgaacctca ccagtatgtc caaaatacta aaatgcgccg gcaatgaaga
     1141 tatcattaca ctaagggccg aagataacgc ggataccttg gcgctagtat ttgaagcacc
     1201 aaaccaggag aaagtttcag actatgaaat gaagttgatg gatttagatg ttgaacaact
     1261 tggaattcca gaacaggagt acagctgtgt agtaaagatg ccttctggtg aatttgcacg
     1321 tatatgccga gatctcagcc atattggaga tgctgttgta atttcctgtg caaaagacgg
     1381 agtgaaattt tctgcaagtg gagaacttgg aaatggaaac attaaattgt cacagacaag
     1441 taatgtcgat aaagaggagg aagctgttac catagagatg aatgaaccag ttcaactaac
     1501 ttttgcactg aggtacctga acttctttac aaaagccact ccactctctt caacggtgac
     1561 actcagtatg tctgcagatg taccccttgt tgtagagtat aaaattgcgg atatgggaca
     1621 cttaaaatac tacttggctc ccaagatcga ggatgaagaa ggatcttagg gatccaccgg
     1681 atctagataa ctgatcataa tcagccatac cacatt
//
