LOCUS       sequence-87369-e        2086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2086)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Emerald-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(47..109,110..1267)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1322..1331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1328..1330,1331..1333,1334..2047)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1373..1394)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1634..1653)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1981..2002
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc gccaccatgg cggccccggg
       61 cgcccggcgt ccgctgctcc tgttgctgct ggcaggcctt gcacacagcg ccccagcact
      121 gttcgaggtg aaagacaaca acggcacagc gtgtataatg gccagcttct ctgcctcctt
      181 tctgaccacc tatgaggctg gacatgtttc taaggtctcg aatatgaccc tgccagcctc
      241 tgcagaagtc ctgaagaata gcagctcttg tggtgaaaag aatgcttctg agcccaccct
      301 cgcaatcacc tttggagaag gatatttact gaaactcacc ttcacaaaaa acacaacacg
      361 ttacagtgtc cagcacatgt atttcacata taacctgtca gacacacaat tctttcccaa
      421 tgccagctcc aaagggcccg acactgtgga ttccacaact gacatcaagg cagacatcaa
      481 caaaacatac cgatgtgtca gcgacatcag ggtctacatg aagaatgtga ccattgtgct
      541 ctgggacgct actatccagg cctacctgcc gagtagcaac ttcagcaagg aagagacacg
      601 ctgcccacag gatcaacctt ccccaactac tgggccaccc agcccctcac caccacttgt
      661 gcccacaaac cccagtgtgt ccaagtacaa tgtgactggt gacaatggaa cctgcctgct
      721 ggcctctatg gcactgcaac tcaacatcac ctacatgaag aaggacaaca cgactgtgac
      781 cagagcattc aacatcaacc caagtgacaa atatagtggg acttgcggtg cccagttggt
      841 gaccctgaag gtggggaaca agagcagagt cctggagctg cagtttggga tgaatgccac
      901 ttctagcctg tttttcctgc aaggagttca gttgaacatg actcttcctg atgccataga
      961 gcccacgttc agcacctcca actattccct gaaagctctt caggccagtg tcggcaactc
     1021 atacaagtgc aacagtgagg agcacatctt tgtcagcaag gcgctcgccc tcaatgtctt
     1081 cagcgtgcaa gtccaggctt tcagggtaga aagtgacagg tttgggtctg tggaagagtg
     1141 tgtacaggac ggtaacaaca tgctgatccc cattgctgtg ggcggggccc tggcagggct
     1201 ggtcctcatc gtcctcatcg cctacctcat cggcaggaag aggagtcacg cgggctatca
     1261 gaccatctcg gaattcggct ccaccggctc caccggctcc accggcgcgg atccaccggt
     1321 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1381 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1441 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1501 gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct accccgacca
     1561 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1621 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1681 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1741 ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg ccgacaagca
     1801 gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg gcagcgtgca
     1861 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1921 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1981 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2041 caagtaagcg gccgcgactc tagatcataa tcagccatac cacatt
//
