LOCUS       sequence-87371-e        2426 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2426)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2426
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..88
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      1661..1670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1667..1669,1670..1672,1673..2386)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1712..1733)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2320..2341
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcatgg ggagcagcaa
       61 gagcaagccc aaggacccca gccagcgccg gcgcagcctg gagccacccg acagcaccca
      121 ccacggggga ttcccagcct cgcagacccc caacaagaca gcagcccccg acacgcaccg
      181 cacccccagc cgctcctttg ggaccgtggc caccgagccc aagctcttcg ggggcttcaa
      241 cacttctgac accgtcacgt cgccgcagcg tgccggggca ctggctggcg gcgtcaccac
      301 tttcgtggct ctctatgact acgagtcccg gactgaaacg gacttgtcct tcaagaaagg
      361 agaacgcctg cagattgtca acaacacgga aggtgactgg tggctggctc attccctcac
      421 tacaggacag acgggctaca tccccagtaa ctatgtcgcg ccctcagact ccatccaggc
      481 tgaagagtgg tactttggga agatcactcg tcgggagtcc gagcggctgc tgctcaaccc
      541 cgaaaacccc cggggaacct tcttggtccg ggagagcgag acgacaaaag gtgcctattg
      601 cctctccgtt tctgactttg acaacgccaa ggggctcaat gtgaagcact acaagatccg
      661 caagctggac agcggcggct tctacatcac ctcacgcaca cagttcagca gcctgcagca
      721 gctggtggcc tactactcca aacatgctga tggcttgtgc caccgcctga ccaacgtctg
      781 ccccacgtcc aagccccaga cccagggact cgccaaggac gcgtgggaaa tcccccggga
      841 gtcgctgcgg ctggaggtga agctggggca gggctgcttt ggagaggtct ggatggggac
      901 ctggaacggc accaccagag tggccataaa gactctgaag cccggcacca tgtccccgga
      961 ggccttcctg caggaagccc aagtgatgaa gaagctccgg catgagaagc tggttcagct
     1021 gtacgcagtg gtgtcggaag agcccatcta catcgtcact gagtacatga gcaaggggag
     1081 cctcctggat ttcctgaagg gagagatggg caagtacctg cggctgccac agctcgtcga
     1141 tatggctgct cagattgcat ccggcatggc ctatgtggag aggatgaact acgtgcaccg
     1201 agacctgcgg gcggccaaca tcctggtggg ggagaacctg gtgtgcaagg tggctgactt
     1261 tgggctggca cgcctcatcg aggacaacga gtacacagca cggcaaggtg ccaagttccc
     1321 catcaagtgg acagcccccg aggcagccct ctatggccgg ttcaccatca agtcggatgt
     1381 ctggtccttc ggcatcctgc tgactgagct gaccaccaag ggccgggtgc catacccagg
     1441 gatggtcaac agggaggtgc tggaccaggt ggagaggggc taccgcatgc cctgcccgcc
     1501 cgagtgcccc gagtcgctgc atgacctcat gtgccagtgc tggcggaagg accctgagga
     1561 gcggcccact tttgagtacc tgcaggcctt cctggaggac tacttcacct cgacagagcc
     1621 ccagtaccag cctggagaga acctacggga tccaccggtc gccaccatgg tgagcaaggg
     1681 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1741 ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc accaacggca agctgaccct
     1801 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1861 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcgcc acgacttctt
     1921 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcagcttca aggacgacgg
     1981 cacctacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2041 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2101 cttcaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     2161 cttcaagatc cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca
     2221 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     2281 gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2341 gaccgccgcc gggatcactc acggcatgga cgagctgtac aagtaagcgg ccgcgactct
     2401 agatcataat cagccatacc acattt
//
