LOCUS       sequence-87374-e        1691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 485, Emission: 
            507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1691)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-EB3-7
FEATURES             Location/Qualifiers
     source          1..1691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      926..935
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(932..934,935..937,938..1651)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(977..998)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1585..1606
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
       61 tgccaccatg gccgtcaatg tgtactccac atctgtgacc agtgaaaatc tgagtcgcca
      121 tgatatgctt gcatgggtca acgactccct gcacctcaac tataccaaga tagaacagct
      181 ttgttcaggg gcagcctact gccagttcat ggacatgctc ttccccggct gtgtgcactt
      241 gaggaaagtg aagttccagg ccaaactaga gcatgaatac atccacaact tcaaggtgct
      301 gcaagcagct ttcaagaaga tgggtgttga caaaatcatt cctgtagaga aattagtgaa
      361 aggaaaattc caagataatt ttgagtttat tcagtggttt aagaaattct ttgacgcaaa
      421 ctatgatgga aaggattaca accctctgct ggcgcggcag ggccaggacg tagcgccacc
      481 tcctaaccca ggtgatcaga tcttcaacaa atccaagaaa ctcattggca cagcagttcc
      541 acagaggacg tcccccacag gcccaaaaaa catgcagacc tctggccggc tgagcaatgt
      601 ggcccccccc tgcattctcc ggaagaatcc tccatcagcc cgaaatggcg gccatgagac
      661 tgatgcccaa attcttgaac tcaaccaaca gctggtggac ttgaagctga cagtggatgg
      721 gctggagaag gaacgtgact tctacttcag caaacttcgt gacatcgagc tcatctgcca
      781 ggagcatgaa agtgaaaaca gccctgttat ctcaggcatc attggcatcc tctatgccac
      841 agaggaagga ttcgcacccc ctgaggacga tgagattgaa gagcatcaac aagaagacca
      901 ggacgagtac ctggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
      961 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1021 gcgcggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
     1081 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     1141 gtgcttcagc cgctaccccg accacatgaa gcgccacgac ttcttcaagt ccgccatgcc
     1201 cgaaggctac gtccaggagc gcaccatcag cttcaaggac gacggcacct acaagacccg
     1261 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1321 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
     1381 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     1441 caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1501 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg tgctgagcaa
     1561 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1621 cactcacggc atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc
     1681 ataccacatt t
//
