LOCUS       sequence-87376-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-ER-5
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..95
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     46..66
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      190..199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(196..198,199..201,202..912)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(241..262)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     849..870
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caaatcaacg gactttccaa aatgtcgtac actccgcccc attgacgcaa atgggcggta
       61 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      121 agcgccacca tgctgctatc cgtgccgttg ctgctcggcc tcctcggcct ggccgtcgcc
      181 gcaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      241 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgcgcgg cgagggcgag
      301 ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      361 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      421 cccgaccaca tgaagcgcca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      481 gagcgcacca tcagcttcaa ggacgacggc acctacaaga cccgcgccga ggtgaagttc
      541 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      601 aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacgtcta tatcaccgcc
      661 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacgt ggaggacggc
      721 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      781 ctgcccgaca accactacct gagcacccag tccgtgctga gcaaagaccc caacgagaag
      841 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactca cggcatggac
      901 gagctgtaca agtccggaaa ggacgagctg taagaattct gcagtcgacg gtaccgcggg
      961 cccgggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt
     1021 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaa
//
