LOCUS       sequence-87379-e        1169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1169)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-H2B-6
FEATURES             Location/Qualifiers
     source          1..1169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..390
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(409..411,412..414,415..1128)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(454..475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1062..1083
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaccgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa gggctccaag
       61 aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag
      121 agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac cggcatttcg
      181 tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt
      241 gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag ggagatccag
      301 acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc cgagggtact
      361 aaggccatca ccaagtacac cagcgctaag gatccaccgg tcgccaccat ggtgagcaag
      421 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      481 ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg caagctgacc
      541 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      601 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagcg ccacgacttc
      661 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcagctt caaggacgac
      721 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      781 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      841 aacttcaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      901 aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag
      961 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1021 cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1081 gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaagtaagc ggccgcgact
     1141 ctagatcata atcagccata ccacatgag
//
