LOCUS       sequence-87380-e        2085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2085)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(45..107,108..1265)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1320..1329
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1326..1328,1329..1331,1332..2045)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1371..1392)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1979..2000
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagcgc caccatggcg gccccgggcg
       61 cccggcgtcc gctgctcctg ttgctgctgg caggccttgc acacagcgcc ccagcactgt
      121 tcgaggtgaa agacaacaac ggcacagcgt gtataatggc cagcttctct gcctcctttc
      181 tgaccaccta tgaggctgga catgtttcta aggtctcgaa tatgaccctg ccagcctctg
      241 cagaagtcct gaagaatagc agctcttgtg gtgaaaagaa tgcttctgag cccaccctcg
      301 caatcacctt tggagaagga tatttactga aactcacctt cacaaaaaac acaacacgtt
      361 acagtgtcca gcacatgtat ttcacatata acctgtcaga cacacaattc tttcccaatg
      421 ccagctccaa agggcccgac actgtggatt ccacaactga catcaaggca gacatcaaca
      481 aaacataccg atgtgtcagc gacatcaggg tctacatgaa gaatgtgacc attgtgctct
      541 gggacgctac tatccaggcc tacctgccga gtagcaactt cagcaaggaa gagacacgct
      601 gcccacagga tcaaccttcc ccaactactg ggccacccag cccctcacca ccacttgtgc
      661 ccacaaaccc cagtgtgtcc aagtacaatg tgactggtga caatggaacc tgcctgctgg
      721 cctctatggc actgcaactc aacatcacct acatgaagaa ggacaacacg actgtgacca
      781 gagcattcaa catcaaccca agtgacaaat atagtgggac ttgcggtgcc cagttggtga
      841 ccctgaaggt ggggaacaag agcagagtcc tggagctgca gtttgggatg aatgccactt
      901 ctagcctgtt tttcctgcaa ggagttcagt tgaacatgac tcttcctgat gccatagagc
      961 ccacgttcag cacctccaac tattccctga aagctcttca ggccagtgtc ggcaactcat
     1021 acaagtgcaa cagtgaggag cacatctttg tcagcaaggc gctcgccctc aatgtcttca
     1081 gcgtgcaagt ccaggctttc agggtagaaa gtgacaggtt tgggtctgtg gaagagtgtg
     1141 tacaggacgg taacaacatg ctgatcccca ttgctgtggg cggggccctg gcagggctgg
     1201 tcctcatcgt cctcatcgcc tacctcatcg gcaggaagag gagtcacgcg ggctatcaga
     1261 ccatctcgga attcggctcc accggctcca ccggctccac cggcgcggat ccaccggtcg
     1321 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1381 tggacggcga cgtaaacggc cacaagttca gcgtgcgcgg cgagggcgag ggcgatgcca
     1441 ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1501 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     1561 tgaagcgcca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1621 tcagcttcaa ggacgacggc acctacaaga cccgcgccga ggtgaagttc gagggcgaca
     1681 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1741 ggcacaagct ggagtacaac ttcaacagcc acaacgtcta tatcaccgcc gacaagcaga
     1801 agaacggcat caaggccaac ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc
     1861 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1921 accactacct gagcacccag tccgtgctga gcaaagaccc caacgagaag cgcgatcaca
     1981 tggtcctgct ggagttcgtg accgccgccg ggatcactca cggcatggac gagctgtaca
     2041 agtaagcggc cgcgactcta gatcataatc agccatacca cattt
//
