LOCUS       sequence-87382-e        2210 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 485, Emission: 
            507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2210)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2210
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1456..1465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1462..1464,1465..1467,1468..2181)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1507..1528)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2115..2136
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgctaccgg actcagatct cgagctcaag cttcgaattc ccatgtccac caggtccgtg
       61 tcctcgtcct cctaccgcag gatgttcggc ggcccgggca ccgcgagccg gccgagctcc
      121 agccggagct acgtgactac gtccacccgc acctacagcc tgggcagcgc gctgcgcccc
      181 agcaccagcc gcagcctcta cgcctcgtcc ccgggcggcg tgtatgccac gcgctcctct
      241 gccgtgcgcc tgcggagcag cgtgcccggg gtgcggctcc tgcaggactc ggtggacttc
      301 tcgctggccg acgccatcaa caccgagttc aagaacaccc gcaccaacga gaaggtggag
      361 ctgcaggagc tgaatgaccg cttcgccaac tacatcgaca aggtgcgctt cctggagcag
      421 cagaataaga tcctgctggc cgagctcgag cagctcaagg gccaaggcaa gtcgcgcctg
      481 ggggacctct acgaggagga gatgcgggag ctgcgccggc aggtggacca gctaaccaac
      541 gacaaagccc gcgtcgaggt ggagcgcgac aacctggccg aggacatcat gcgcctccgg
      601 gagaaattgc aggaggagat gcttcagaga gaggaagccg aaaacaccct gcaatctttc
      661 agacaggatg ttgacaatgc gtctctggca cgtcttgacc ttgaacgcaa agtggaatct
      721 ttgcaagaag agattgcctt tttgaagaaa ctccacgaag aggaaatcca ggagctgcag
      781 gctcagattc aggaacagca tgtccaaatc gatgtggatg tttccaagcc tgacctcacg
      841 gctgccctgc gtgacgtacg tcagcaatat gaaagtgtgg ctgccaagaa cctgcaggag
      901 gcagaagaat ggtacaaatc caagtttgct gacctctctg aggctgccaa ccggaacaat
      961 gacgccctgc gccaggcaaa gcaggagtcc actgagtacc ggagacaggt gcagtccctc
     1021 acctgtgaag tggatgccct taaaggaacc aatgagtccc tggaacgcca gatgcgtgaa
     1081 atggaagaga actttgccgt tgaagctgct aactaccaag acactattgg ccgcctgcag
     1141 gatgagattc agaatatgaa ggaggaaatg gctcgtcacc ttcgtgaata ccaagacctg
     1201 ctcaatgtta agatggccct tgacattgag attgccacct acaggaagct gctggaaggc
     1261 gaggagagca ggatttctct gcctcttcca aacttttcct ccctgaacct gagggaaact
     1321 aatctggatt cactccctct ggttgatacc cactcaaaaa ggacacttct gattaagacg
     1381 gttgaaacta gagatggaca ggttatcaac gaaacttctc agcatcacga tgaccttgaa
     1441 ggggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     1501 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gcgcggcgag
     1561 ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag
     1621 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1681 cgctaccccg accacatgaa gcgccacgac ttcttcaagt ccgccatgcc cgaaggctac
     1741 gtccaggagc gcaccatcag cttcaaggac gacggcacct acaagacccg cgccgaggtg
     1801 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1861 gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa cgtctatatc
     1921 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacgtggag
     1981 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2041 gtgctgctgc ccgacaacca ctacctgagc acccagtccg tgctgagcaa agaccccaac
     2101 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactcacggc
     2161 atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc
//
