LOCUS       sequence-87386-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mWasabi-Actin-7
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaccac
       61 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
      121 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
      181 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
      241 gttcagttac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
      301 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
      361 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
      421 caagggcgag aacttccccc ccaacggccc cgtgatgcag aaggagacca ccggctggga
      481 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagatgaa
      541 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
      601 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
      661 cgacaaggac tacaacaagg tgaccgttta cgagatcgcc gtggcccgca actccaccga
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctatggatg atgatatcgc
      781 cgcgctcgtc gtcgacaacg gctccggcat gtgcaaggcc ggcttcgcgg gcgacgatgc
      841 cccccgggcc gtcttcccct ccatcgtggg gcgccccagg caccagggcg tgatggtggg
      901 catgggtcag aaggattcct atgtgggcga cgaggcccag agcaagagag gcatcctcac
      961 cctgaagtac cccatcgagc acggcatcgt caccaactgg gacgacatgg agaaaatctg
     1021 gcaccacacc ttctacaatg agctgcgtgt ggctcccgag gagcaccccg tgctgctgac
     1081 cgaggccccc ctgaacccca aggccaaccg cgagaagatg acccagatca tgtttgagac
     1141 cttcaacacc ccagccatgt acgttgctat ccaggctgtg ctatccctgt acgcctctgg
     1201 ccgtaccact ggcatcgtga tggactccgg tgacggggtc acccacactg tgcccatcta
     1261 cgaggggtat gccctccccc atgccatcct gcgtctggac ctggctggcc gggacctgac
     1321 tgactacctc atgaagatcc tcaccgagcg cggctacagc ttcaccacca cggccgagcg
     1381 ggaaatcgtg cgtgacatta aggagaagct gtgctacgtc gccctggact tcgagcaaga
     1441 gatggccacg gctgcttcca gctcctccct ggagaagagc tacgagctgc ctgacggcca
     1501 ggtcatcacc attggcaatg agcggttccg ctgccctgag gcactcttcc agccttcctt
     1561 cctgggcatg gagtcctgtg gcatccacga aactaccttc aactccatca tgaagtgtga
     1621 cgtggacatc cgcaaagacc tgtacgccaa cacagtgctg tctggcggca ccaccatgta
     1681 ccctggcatt gccgacagga tgcagaagga gatcactgcc ctggcaccca gcacaatgaa
     1741 gatcaagatc attgctcctc ctgagcgcaa gtactccgtg tggatcggcg gctccatcct
     1801 ggcctcgctg tccaccttcc agcagatgtg gatcagcaag caggagtatg acgagtccgg
     1861 cccctccatc gtccaccgca aatgcttcta gggatccacc ggatctagat aactgat
//
