LOCUS       sequence-8739-ex       10633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Martin VJ, Pitera DJ, Withers ST, Newman JD, Keasling JD
  TITLE     Engineering a mevalonate pathway in Escherichia coli for production 
            of terpenoids.
  JOURNAL   Nat Biotechnol. 2003 Jul . 21(7):796-802.
  PUBMED    12778056
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2003 Jul . 21(7):796-802."
COMMENT     Sequence Label: pMBIS
FEATURES             Location/Qualifiers
     source          1..10633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3147..3169
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3161..3178
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3188..3207
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4151..4167
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4151..4167
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(5660..5671)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8694..8710)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8695..8711)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8740..8760)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8740..8758)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8779..8795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8779..8795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8792..8814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8803..8819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8827..8833,8834..8851,8852..8857))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8872..8893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(9087..10277)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     9906..9925
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(10325..10330,10331..10347,10348..10353))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(10380..10398)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc ttatttatta cgctggatga
     3241 tgtagtccgc tagcgcttcc agtgccgagg tatcgagtga ctgttcagcc agttgtttca
     3301 gcgactgacg ggcatcgtcg atcagatccc gggctttctt ccgggcttgc tcaagaccca
     3361 gaagtgcagg gtaggtactt ttaccaagtt gctggtcggc accctggcgt tttcccaacg
     3421 ttgcagtatc tcccaccaca tccaggatgt catcctgaac ctggaaggca aggccgatgc
     3481 tctctgcata cttgtcgagt accggcagag cacgacgtcc tttatctccg gcgcttaatg
     3541 caccaaggcg aacggcggcg cgaatcaatg cgccggtttt atgacgatga atacgctcaa
     3601 gcgcgtccag aggtacgtgt ttgccttccg cgtctaaatc taatgcctga ccaccgcaca
     3661 ttccggcaat accactggcg ctcgccagtt cagaaatcat cgaaattctg tcgcggtccg
     3721 acacttccgg catatcggca tcgcttaaaa tcgagaacgc cagcgtttgt aaagcgtcgc
     3781 cagcgagaat cgcgtttgct tcgccaaact tcacatggca ggttggcaaa ccgcgacgca
     3841 gatcgtcatc atccattgcc ggtaaatcat catgaattaa tgagtaagcg tggatacact
     3901 caacggcggc agcgggtgcg tccagcgtgt ttgtgctaac gccgaacata tgaccggtgg
     3961 cataaaccag gaaaggtcgc aggcgcttac cacctaataa tgcgccatac tgcatggttt
     4021 cgaccacggg agtgttctga aagggcagtg gggcgataaa acggctcagc gcctggttgg
     4081 cctgcttaac gcaggcttcg agttgctgcg gaaagtccat tactcattcc tcctccgcgg
     4141 tggcggccgc tctagaacta gtggatcccc cgggttattt aagctgggta aatgcagata
     4201 atcgttttct ggcttcgcga tttgtcgcct gcatcaccat ccacggactg aacgcccacg
     4261 gcgtggcatc aataccgtgt aatacatctg ctaaatcaca ccattgataa tccatcactt
     4321 catcatcatt gatctgtaac gcactagtgg tgcgtgcggc aaataccgga cacacttcat
     4381 tttccacaat gccactcgga tcggtggcgc ggtagcgaaa gtcaggatag atagattcag
     4441 gaggcgtaat ttccacgcca agctcataac ggcaacggcg gatcactgcg tcttcgttgc
     4501 tttctcccag ttgtgggtgc ccacaaaccg agttagtcca cacgccaggc catgcttttt
     4561 tgctcagtgc gcggcgggta actaataatt gtcctttggc attaaacagc caactggaga
     4621 acgcgagatg taagcgggtg tctgccgtgt gtgcggcata cttttccagc gtacccgtgg
     4681 gaactccctg tgcattcaat aaaatgacgt gttccgtttg catatagtaa tcctcctccc
     4741 gggctgcagt tattcctttg gtagaccagt ctttgcgtca atcaaagatt cgtttgtttc
     4801 ttgtgggcct gaaccgactt gagttaaaat cactctggca acatcctttt gcaactcaag
     4861 atccaattca cgtgcagtaa agttagatga ttcaaattga tggttgaaag cctcaagctg
     4921 ctcagtagta aatttcttgt cccatccagg aacagagcca aacaatttat agataaatgc
     4981 aaagagtttc gactcatttt cagctaagta gtacaacaca gcatttggac ctgcatcaaa
     5041 cgtgtatgca acgattgttt ctccgtaaaa ctgattaatg gtgtggcacc aactgatgat
     5101 acgcttggaa gtgtcattca tgtagaatat tggagggaaa gagtccaaac atgtggcatg
     5161 gaaagagttg gaatccatca ttgtttcctt tgcaaaggtg gcgaaatctt tttcaacaat
     5221 ggctttacgc atgacttcaa atctctttgg tacgacatgt tcaattcttt ctttaaatag
     5281 ttcggaggtt gccacggtca attgcatacc ctgagtggaa ctcacatcct ttttaatatc
     5341 gctgacaact aggacacaag ctttcatctg aggccagtca gagctgtctg cgatttgtac
     5401 tgccatggaa tcatgaccat cttcagcttt tcccatttcc caggccacgt atccgccaaa
     5461 caacgatcta caagctgaac cagacccctt tcttgctatt ctagatattt ctgaagttga
     5521 ctgtggtaat tggtataact tagcaattgc agagaccaat gcagcaaagc cagcagcgga
     5581 ggaagctaaa ccagctgctg taggaaagtt attttcggag acaatgtgga gtttccattg
     5641 agataatgtg ggcaatgagg cgtccttcga ttccatttcc tttcttaatt ggcgtaggtc
     5701 gcgcagacaa ttttgagttc tttcattgtc gatgctgtgt ggttctccat ttaaccacaa
     5761 agtgtcgcgt tcaaactcag gtgcagtagc cgcagaggtc aacgttctga ggtcatcttg
     5821 cgataaagtc actgatatgg acgaattggt gggcagattc aacttcgtgt cccttttccc
     5881 ccaatactta agggttgcga tgttgacggg tgcggtaacg gatgctgtgt aaacggtcat
     5941 gagtattacc tcctatttat caagataagt ttccggatct ttttctttcc taacacccca
     6001 gtcagcctga gttacatcca gccattgaac cttagaaaat cttttgtcat tagcggtttg
     6061 agccctaaga tcaacatctt gcttagtaat cactgcaatg gcgtcataac caccagcacc
     6121 aggtattaag caagtaagaa ctccttttaa ggtctggcaa tcatccaata agctagtttg
     6181 tacgggaggt tcgatatcgg caccagattc tttagttatt tttctaaagg aacgtctaat
     6241 tgtggcaact gcatctctaa cttctgtgat ttcaggatac ttttgacagg tacagtcatt
     6301 cctctcaaga gactcaaata tctgatcgct gtaatcgtca tgagtctcgt gtaagcgatc
     6361 tagtttagat agtccatcca taaatctaga atttgcatga tcgagttctg tatatatttt
     6421 caagctttct ggcatatgcg aatcatacca attttttacc ttctggacca gttttactgt
     6481 ttctgaacca ttcttaatat cgcccatcca taaagttaat cccgaaggta aatggttact
     6541 tttaatcgta atattccagt cttcttcatc aaccaaatgc gccagtttac tgccgtaagt
     6601 agcacttcca atatctggca aattagagat taatgcgggt gggaatcttc tatatctgat
     6661 agatccatat gctgccgccg ctacatcaaa cccgcttcca attttaccct gagcttgaca
     6721 atgagcaact tgtgctaaat tatgaataac ttctctatat ttgtctacat tattttccag
     6781 gtccgataca aaaaaggagg ccaaagctgt agttaaaact gtgactaaac ctgccgagga
     6841 gcccagccct gttttgggaa cttcttcaat tctgtgcgaa tgaaaactca atcttctgtt
     6901 gccacgatgt tcggtaacgc tatcctcctg agaatggtag gcatcatcag agaaaatatc
     6961 aataacgaac aagtttctat tgcagtagtc gtccatgtta ggtttaaagt agctaaatac
     7021 gttagcgata actttttcaa tgaaagggtt cttagatccg cctatcgaaa caggaatgaa
     7081 gccactttta ggacttatat ggtacagcca ctccccatct ttaaattgtt tacttttcac
     7141 acgcacttca aacttatcag acccttgcaa tgaaccgtaa ggatgggcta cagcatgcat
     7201 tcttgccgat aatccgacta caaatgcttc atattttgta tctaaaacta aatatccacc
     7261 agctagtaac gctttccctg gggcactgaa ggctctcaac tctgacattt gatctgcctc
     7321 ctatgaagtc catggnaaat tcgtgtttcc tggcaataat agatcgtcaa tttgttgctt
     7381 tgtggtagtt ttattttcaa ataattggaa tactagggat ttgattttaa gatctttatt
     7441 caaatttttt gcgcttaaca aacagcagcc agtcccaccc aagtctgttt caaatgtctc
     7501 gtaactaaaa tcatcttgca atttcttttt gaagctgtca atttgctctt gagtaatgtc
     7561 tcttcgtaac aaagtcaaag agcaaccgcc gccaccagca ccggtaagtt ttgtggagcc
     7621 aattctcaaa tcatcgctca gatttttaat aagttctaat ccaggatgag aaacaccgat
     7681 tgagacaagc agtccatgat ttattcttat caattccaat agttgttcat acagttcatt
     7741 attagtttct acagcctcgt catcggtgcc tttacattta cttaacttag tcatgatctc
     7801 taagccttgt agggcacatt cacccatggc atctagaatt ggcttcataa cttcaggaaa
     7861 tttctcggtg accaacacac gaacgcgagc aacaagatct tttgtagacc ttggaattct
     7921 agtataggtt aggatcattg gaatggctgg gaaatcatct aagaacttaa aattgtttgt
     7981 gtttattgtt ccattatgtg agtctttttc aaatagcagg gcattaccat aagtggccac
     8041 agcgttatct attcctgaag gggtaccgtg aatacacttt tcacctatga aggcccattg
     8101 attcactata tgcttatcgt tttctgacag cttttccaag tcattagatc ctattaaccc
     8161 ccccaagtag gccatagcta aggccagtga tacagaaata gaggcgcttg agcccaaccc
     8221 agcaccgatg ggtaaagtag actttaaaga aaacttaata ttcttggcat gggggcatag
     8281 gcaaacaaac atatacagga aacaaaacgc tgcatggtag tggaaggatt cggatagttg
     8341 agctaacaac ggatccaaaa gactaacgag ttcctgagac aagccatcgg tggcttgttg
     8401 agccttggcc aatttttggg agtttacttg atcctcggtg atggcattga aatcattgat
     8461 ggaccactta tgattaaagc taatgtccgg gaagtccaat tcaatagtat ctggtgcaga
     8521 tgactcgctt attagcaggt aggttctcaa cgcagacaca ctagcagcga cggcaggctt
     8581 gttgtacaca gcagagtgtt caccaaaaat aataaccttt cccggtgcag aagttaagaa
     8641 cggtaatgac atggttaatt cctcctactg caggaattcg atatcaagct tatcgatacc
     8701 gtcgacctcg agggggggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
     8761 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8821 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8881 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8941 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcat
     9001 ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc
     9061 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
     9121 cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc
     9181 catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt
     9241 aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc
     9301 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
     9361 atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg
     9421 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg
     9481 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
     9541 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
     9601 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
     9661 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc
     9721 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     9781 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     9841 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     9901 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     9961 gccacgatgc gtccggcgta gaggatccac aggacgggtg tggtcgccat gatcgcgtag
    10021 tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac
    10081 agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg
    10141 ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc
    10201 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
    10261 gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
    10321 ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg
    10381 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
    10441 aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg ggcctgtttc
    10501 tggcgctgga cttcccgctg ttccgtcagc agcttttcgc ccacggcctt gatgatcgcg
    10561 gcggccttgg cctgcatatc ccgattcaac ggccccaggg cgtccagaac gggcttcagg
    10621 cgctcccgaa ggt
//
