LOCUS       sequence-87392-e        2175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2175)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1411..1420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1417..2127
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 tagtgacgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 ggcagcactt ttctttcatt ggggggaagg cgtgaggaaa gtaccaaaca gcagcagact
      121 tttaaacttt aaacagacag gtctgagagc ctgaactctc atttttcctt tgacttcagc
      181 ctccaaggag ttccaccaac tttggcgcgc cggcttcact ttcattaagt gaaagagagg
      241 tgcccagaca tgggtgactg gagtgccttg gggaagcttc tggacaaggt ccaagcctac
      301 tccaccgctg gagggaaggt gtggctgtca gtgctcttca tattcagaat cctgctcctg
      361 gggacagctg ttgagtcagc ttggggtgat gaacagtctg cctttcgctg taacactcaa
      421 caacctggct gcgaaaacgt ctgctatgac aagtccttcc ccatctctca cgtgcgcttc
      481 tgggtccttc agatcatatt cgtgtctgtg cccacactcc tgtacttggc ccatgtgttc
      541 tatgtgatga ggaaggaaga gaagctaaac aagaaagaag aggagctcaa agtggcccag
      601 actgacgggg tcaacgtgga gatgcacctg aagcagattg aaatcaagaa gttcaagtac
      661 gggattgaag agcacggcaa ggtgaaaatg aggggcggct tgctgagaac ctacatcatc
      721 agcatcctct tcaagtctgt cttcgaggtg gccttcctgc tcatccagtg gtacatctat
      781 gggttcagct tgagcgcggt ctacacctgc aagagagatc cctgccccca ccaggtagac
      841 tgcttcctct cacgtcccac ggagaaaacc atcttcatca tcttcatgct ggtggtgtcc
      901 ttggtgtctc tcgctttgaa catcattgag ctcttctacg tcttcttcaa aggcgttaag
      961 gatcgcgtga agggaagaag cgatccttac cacgccacca ctggcccact gagcccatca
     1021 aaagactgcg gatctccaaa atacgcctac ttcaatggct gctcctcacc aacggctcca
     1081 ctctcgccta tgtctcctcc tgggtacaag ctggttactg gtgacagaaa caattcctcg
     1141 tgccgcaatt acaacaagca agctagcgag caaaactggg cgaactacag cgcagagcaa
     1201 aatcgcatgg ggcaggccgg aagcaccatc tccaactcgc acgcccagcc gttcgatttc
     1261 cccgacgaca accagaatgc caaaaaagtt gctgctggac atgaactcca gccattagcc
     1321 atcgtggacc aacgaccttc cagcagagcc agcagccgcg ccagcagcag gcctcggcct
     1381 gatgacctgg agattgcgga tccaccggtc gccaccatgg tgagcaaggg cgaggagacc
     1441 acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat
     1501 ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc
     1561 atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc
     1621 gcgttcagtt acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc
     1681 aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc
     1741 atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac
     1801 ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaaggagac caccggctgg
     1861 gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagatg
     1921 aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc
     1981 aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac
     2041 cacgacaagg actacaacaa ggtgaccgtt tacgagatcg ccgtggcccg caactccacc
     2101 gacggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata
     2161 ccacatgtag agatc
//
