LOCUS       sequence-87393-e        1685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 493, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1685)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mWasabi-EB3-7
FEATURES             Location/Qualifiers
     source          1..1685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             931..1641
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgact
       61 gccaccatgg ccgtcaatgt gtactccaca tctgtgacca gtgaaaatct gagtcgccat
      121 gatatgcttg catgggtcaa cgactccctg cacctcaact ataccaagat agaacagctt
      181 tgttcagggg cagcctactg ccagttcatg gacatgctct tccccggctg tgtgcacttg
      241 aggaaagtga agttccaggc caaactagag catgaataca tccacaactt caaggtgctg
      301 caagcagctt tcaagaagat gggtgttgac aaaatcattc ctgtagagaa attagtgaaa
      361 ggaaaattcc aagataattt tgagtttatt cagtggttta agaaattctt tgacgcaaac
      421 tatgatggaa aggattacaa ccctctgctg gcgcggcagg gccaggacgt agcgccacct
      481 cctaacccag gtgatcagat cttcaacaaa tccaagaaac tcattggcac agcagttcca
      541 cagaggacgt cccccacagg cccaaaaaac atgcagacct ctggccggct gagcaatgtg
      601 gcccccccct gcattctccg gaagaatcct ccatcagccc gaaatggcgg ccatgagact
      661 gatgcccaaa ttcttgaact caaccaacag ctggtggact tgaagctgac agtggatggg
      721 ctggagaagg aacgtgactt ctacttcagc aaacttcgtg acatcgagct catctgccag
      781 gagcatgaaa gtgaaaacag ccctgttatc tcaggcatca ttggcatcct ctatgccaca
      841 gaggaaggat tcgcaccccc tgaggacgat gagattgaag agcatcaaca agaagaccag
      901 gacgagtacc tggatccacc ggtcgccacc atggtgagca agggcgagga gaccacaatg
      961 ggcgtaatca agcccgacat gaagatcaag ctgaagatgg agggcaacgt gaatggccac
     1021 gccttcgtga tcgagggcga gggcgagggc aagccctacg acggcaccaa caccatcaac
     1081 ctggaggtga aggagggagc ccccctgccc ttctcctacg acattctgac caccgcgttc
     1141 agttacggca acagggcctt caccaagtac cccgacgaca tccccaacta cttcaagcag
     1201 tccttccccg agggctactc ttgggagcgc accatgacct tcgaggacaa gggcatcgtg
     1261 aaggtgaagt ccgacatctc catggaggag gactccttca tctacgagat acacctcaag
     1321 ggcgagaact tcccccccaa cggccccgtg atgcagaagg agaccaccgg ctgggacgcc
     1381 tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg gcgacgtcaa gatgaagctg
     1441 ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga ccatctacag ggccaagaag
     1501 gcggtgaagc tgcccgacta tcactttgtg gaccaccgca tcgagatcct gaaccacgac
     1561 aaggactaca acaaggtgac cgtttacgag atcgccgtgg cccgcaactc caccgacggc
     1621 atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
     1681 gtaga
//
