LOCUS       sequence-87394-e        1480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1480)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mWasabi-Endo-14
FEATURES             Location/Qualifiers
     source          1..1480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
     CDS             788..817
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaccac
       61 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
      121 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
      181 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
      241 gttcagttac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
      301 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
      361 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
      421 caagggcgag aacttccccc ccaacggccc cgtgatgcag aaggagacca ccggctggga
      481 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagatgaa
      541 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
      601 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
      661 cgacaaggac tacaacaagg tgaccgttta cgagatcgcc gtggcccgca actccaccga
      721 cggcatggac gagctgtaca agtccggatc agatctcgag ctcaagcttg atatcgaatt
      781 ccccatggag cagaagctga tctccgagga ggacctgaac cggaagaaac tggtggttgt
      841 tggtgatgga gcctgtggaa agacatgctt gctcatagtc ttcagcaagg acgagttccc
      901 agaggtgtat gtgcccacag tgtttgagaa ctatgtggca gatatcgagg tggatggaaa
      961 gcaggtagag ttggctttgt gggacacagc tggccaggag gactacgacc gcctgcggcc
     1021 gctctcctac ccggacaccg acgtcattct catgtgcttc tcggtggaca gcccggactc
     1081 gctggagaac atccccgaga agtgggtccc cgaggtgaag cacttctgtc ccaatgtgcc
     1141 catcatcctg gtggccaaca aaaaagacct gcgcagcgac gagcatgtcc gcacagagct
     1201 ggcccgcatg aagcaggaac ccgttcgcac ggatgacggc cgcgccatgg ccgtgcgcat
     1261 ccaagcctac gactacctcg agtgctctgc caagaccaag gaaggcgtgc gcgaggtctt
     1321 cgagacggcc acgcgcgccg cgctgcagaa gcgctacggc tcccagaacg gctgcatcaa
     1381 ctgctgcaag gtgctatgag ggccgcgccc gtcgcgcctg cccctgccgg ggatccaccg
     1441 gatctagata actgatcata atcagccata ccacattgag
//
